Display options
Cite
Song ZQ, Xu XH, Pan ZH, et al. Mechanisms for steep pulse irreversible electroporation technology to kill human large cell lung cancer cells L9981. Int J Clin Exp Med. 2014;7(8):2386-94
Song, Z. Q., Xu, X. H., Pan, Z. H., Yao, C. G., & Zhou, Q. H. (2014). Mechanisms for steep pulse irreversible electroporation technology to kill human large cell lung cancer cells L9981. International journal of clinical and experimental medicine, 7(8), 2386-94.
Song, Zuo-Qing, et al. "Mechanisms for steep pulse irreversible electroporation technology to kill human large cell lung cancer cells L9981." International journal of clinical and experimental medicine vol. 7,8 (2014): 2386-94.
Song ZQ, Xu XH, Pan ZH, Yao CG, Zhou QH. Mechanisms for steep pulse irreversible electroporation technology to kill human large cell lung cancer cells L9981. Int J Clin Exp Med. 2014 Aug 15;7(8):2386-94. eCollection 2014. PMID: 25232444; PMCID: PMC4161604.
Copy Download .nbib Format: NLM
Share it on

Int J Clin Exp Med. 2014 Aug 15;7(8):2386-94. eCollection 2014.

Mechanisms for steep pulse irreversible electroporation technology to kill human large cell lung cancer cells L9981.

International journal of clinical and experimental medicine

Zuo-Qing Song, Xiao-Hong Xu, Zhen-Hua Pan, Chen-Guo Yao, Qing-Hua Zhou

Affiliations

  1. Department of Lung Cancer Surgery, Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital Tianjin 300052, China.
  2. College of Nursing, Tianjin Medical University Tianjin 300070, China.
  3. College of Electrical Engineering, Chongqing University Chongqing 400044, China.

PMID: 25232444 PMCID: PMC4161604

Abstract

To explore the mechanisms for steep pulse irreversible electroporation technology to kill the lung cancer cell L9981. The apoptosis, cells mitochondrial membrane potential, internal PH changes and the intra-cellular calcium ions concentration were detected after steep pulses acted on the human large cell lung cancer cell L9981. Apoptosis test results indicated that cancer cells mainly experienced necrosis and apoptosis. Along with the increase of electric parameters, the proportion of the necrotic cells increased rapidly; the detection of cells mitochondrial membrane potential indicated that membrane potential occurred depolarization. Steep pulse can cause cancer cells to produce death and apoptosis .The PH value indicated that intracellular PH level down jumped. Internal PH became more acidic and led to cell death. The detection of intra-cellular calcium ions concentration showed that the number of free calcium significantly increased, and this change had killing effects on cell death and apoptosis. Steep pulse could induce cell apoptosis.

Keywords: Steep pulsed; apoptosis; irreversible electrical breakdown; large cell lung cancer; necrosis

References

  1. Thorac Cancer. 2013 Feb;4(1):53-58 - PubMed
  2. Mil Med Res. 2014 Aug 21;1:18 - PubMed
  3. World J Radiol. 2010 Nov 28;2(11):417-24 - PubMed
  4. Thorac Cancer. 2011 Feb;2(1):1-6 - PubMed
  5. Thorac Cancer. 2013 May;4(2):191-194 - PubMed
  6. Eur J Gastroenterol Hepatol. 2009 Jun;21(6):599-605 - PubMed
  7. IEEE Eng Med Biol Mag. 1986;5(4):6-25 - PubMed
  8. Cancer Lett. 2013 May 28;332(2):359-68 - PubMed
  9. Med Oncol. 2010 Jun;27(2):421-9 - PubMed
  10. Thorac Cancer. 2010 May;1(1):35-40 - PubMed
  11. Zhongguo Fei Ai Za Zhi. 2011 Apr;14(4):362-6 - PubMed
  12. J Cell Biochem. 1997 Nov 1;67(2):231-40 - PubMed
  13. Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:130-3 - PubMed
  14. Thorac Cancer. 2012 Feb;3(1):94-97 - PubMed
  15. Drug Resist Updat. 2010 Feb-Apr;13(1-2):2-15 - PubMed
  16. Thorac Cancer. 2011 Aug;2(3):123-127 - PubMed
  17. Curr Opin Urol. 2009 Mar;19(2):188-91 - PubMed
  18. Asia Pac J Oncol Nurs. 2014 Apr-Jun;1(1):46-49 - PubMed
  19. Thorac Cancer. 2013 May;4(2):102-108 - PubMed
  20. CA Cancer J Clin. 2008 Mar-Apr;58(2):71-96 - PubMed
  21. Mol Biotechnol. 2009 Mar;41(3):286-95 - PubMed
  22. Thorac Cancer. 2012 Feb;3(1):34-39 - PubMed

Publication Types