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Bessega CF, Pometti CL, Miller JT, et al. New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae). Appl Plant Sci. 2013;1(5)doi: 10.3732/apps.1200324.
Bessega, C. F., Pometti, C. L., Miller, J. T., Watts, R., Saidman, B. O., & Vilardi, J. C. (2013). New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae). Applications in plant sciences, 1(5), . https://doi.org/10.3732/apps.1200324
Bessega, Cecilia F, et al. "New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae)." Applications in plant sciences vol. 1,5 (2013). doi: https://doi.org/10.3732/apps.1200324
Bessega CF, Pometti CL, Miller JT, Watts R, Saidman BO, Vilardi JC. New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae). Appl Plant Sci. 2013 Apr 23;1(5). doi: 10.3732/apps.1200324. eCollection 2013 May. PMID: 25202541; PMCID: PMC4105034.
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Appl Plant Sci. 2013 Apr 23;1(5). doi: 10.3732/apps.1200324. eCollection 2013 May.

New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae).

Applications in plant sciences

Cecilia F Bessega, Carolina L Pometti, Joe T Miller, Richard Watts, Beatriz O Saidman, Juan C Vilardi

Affiliations

  1. Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
  2. Centre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, Australia.

PMID: 25202541 PMCID: PMC4105034 DOI: 10.3732/apps.1200324

Abstract

PREMISE OF THE STUDY: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis. •

METHODS AND RESULTS: Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia, P. flexuosa, P. kuntzei, P. glandulosa, and P. pallida. •

CONCLUSIONS: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.

Keywords: Fabaceae; Prosopis; microsatellite; next-generation sequencing data

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