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Villani TS, Koroch AR, Simon JE. An improved clearing and mounting solution to replace chloral hydrate in microscopic applications. Appl Plant Sci. 2013;1(5)doi: 10.3732/apps.1300016.
Villani, T. S., Koroch, A. R., & Simon, J. E. (2013). An improved clearing and mounting solution to replace chloral hydrate in microscopic applications. Applications in plant sciences, 1(5), . https://doi.org/10.3732/apps.1300016
Villani, Thomas S, et al. "An improved clearing and mounting solution to replace chloral hydrate in microscopic applications." Applications in plant sciences vol. 1,5 (2013). doi: https://doi.org/10.3732/apps.1300016
Villani TS, Koroch AR, Simon JE. An improved clearing and mounting solution to replace chloral hydrate in microscopic applications. Appl Plant Sci. 2013 May 07;1(5). doi: 10.3732/apps.1300016. eCollection 2013 May. PMID: 25202549; PMCID: PMC4105042.
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Appl Plant Sci. 2013 May 07;1(5). doi: 10.3732/apps.1300016. eCollection 2013 May.

An improved clearing and mounting solution to replace chloral hydrate in microscopic applications.

Applications in plant sciences

Thomas S Villani, Adolfina R Koroch, James E Simon

Affiliations

  1. New Use Agriculture and Natural Plant Products Program, School of Environmental and Biological Sciences, and the New Jersey Agricultural Experiment Station (NJAES), Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, New Jersey 08901 USA ; Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854 USA.
  2. Science Department, City University of New York, Borough of Manhattan Community College, 199 Chambers Street, New York, New York 10007 USA.
  3. New Use Agriculture and Natural Plant Products Program, School of Environmental and Biological Sciences, and the New Jersey Agricultural Experiment Station (NJAES), Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, New Jersey 08901 USA.

PMID: 25202549 PMCID: PMC4105042 DOI: 10.3732/apps.1300016

Abstract

PREMISE OF THE STUDY: This study presents Visikol™, a new proprietary formulation that can be used as an efficient replacement for chloral hydrate as a clearing agent for microscopic examination. In the United States, chloral hydrate is regulated and therefore difficult to acquire. •

METHODS AND RESULTS: Fresh and dry samples of the following plants: ginger (Zingiber officinale), maté (Ilex paraguariensis), lime basil (Ocimum americanum), oregano (Origanum vulgare), and mouse-ear cress (Arabidopsis thaliana), were cleared using Visikol or chloral hydrate solution and compared using a light microscope. •

CONCLUSIONS: This new method can be used successfully to clear specimens, allowing identification of diagnostic characteristics for the identification of plant materials. Visikol is as effective as chloral hydrate in providing clarity and resolution of all tissues examined. Tissues become transparent, allowing observation of deeper layers of cells and making it effective in research, botanical and quality control, and for educational applications.

Keywords: Visikol; acidified chloral hydrate; botanical quality control; clearing; histology; microscopy

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