Display options
Cite
Martins AR, Abreu AG, Bajay MM, et al. Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species. Appl Plant Sci. 2013;1(6)doi: 10.3732/apps.1200507.
Martins, A. R., Abreu, A. G., Bajay, M. M., Villela, P. M., Batista, C. E., Monteiro, M., Alves-Pereira, A., Figueira, G. M., Pinheiro, J. B., Appezzato-da-Glória, B., & Zucchi, M. I. (2013). Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species. Applications in plant sciences, 1(6), . https://doi.org/10.3732/apps.1200507
Martins, Aline R, et al. "Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species." Applications in plant sciences vol. 1,6 (2013). doi: https://doi.org/10.3732/apps.1200507
Martins AR, Abreu AG, Bajay MM, Villela PM, Batista CE, Monteiro M, Alves-Pereira A, Figueira GM, Pinheiro JB, Appezzato-da-Glória B, Zucchi MI. Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species. Appl Plant Sci. 2013 May 16;1(6). doi: 10.3732/apps.1200507. eCollection 2013 Jun. PMID: 25202555; PMCID: PMC4105028.
Copy Download .nbib Format: NLM
Share it on

Appl Plant Sci. 2013 May 16;1(6). doi: 10.3732/apps.1200507. eCollection 2013 Jun.

Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.

Applications in plant sciences

Aline R Martins, Aluana G Abreu, Miklos M Bajay, Priscilla M S Villela, Carlos E A Batista, Mariza Monteiro, Alessandro Alves-Pereira, Glyn M Figueira, José B Pinheiro, Beatriz Appezzato-da-Glória, Maria I Zucchi

Affiliations

  1. Departamento de Ciências Biológicas, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
  2. Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
  3. Departmento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
  4. Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agricolas (CPQBA), Universidade Estadual de Campinas (UNICAMP), C.P. 6171, 13083-970 Campinas, São Paulo, Brazil.
  5. Agência Paulista de Tecnologia dos Agronegócios, Rodovia SP 127, Vila Fátima, 13400-970 Piracicaba, São Paulo, Brazil.

PMID: 25202555 PMCID: PMC4105028 DOI: 10.3732/apps.1200507

Abstract

PREMISE OF THE STUDY: A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. •

METHODS AND RESULTS: Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. •

CONCLUSIONS: The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.

Keywords: Smilax; medicinal plant; microsatellites; sarsaparilla; transferability

References

  1. Methods Mol Biol. 2000;132:365-86 - PubMed

Publication Types