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Pan Y, Pan L, Chen L, et al. Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock. Appl Plant Sci. 2013;1(7)doi: 10.3732/apps.1200004.
Pan, Y., Pan, L., Chen, L., Zhang, L., Nevo, E., & Peng, J. (2013). Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock. Applications in plant sciences, 1(7), . https://doi.org/10.3732/apps.1200004
Pan, Yue, et al. "Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock." Applications in plant sciences vol. 1,7 (2013). doi: https://doi.org/10.3732/apps.1200004
Pan Y, Pan L, Chen L, Zhang L, Nevo E, Peng J. Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock. Appl Plant Sci. 2013 Jun 21;1(7). doi: 10.3732/apps.1200004. eCollection 2013 Jul. PMID: 25202562; PMCID: PMC4103125.
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Appl Plant Sci. 2013 Jun 21;1(7). doi: 10.3732/apps.1200004. eCollection 2013 Jul.

Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock.

Applications in plant sciences

Yue Pan, Lei Pan, Liang Chen, Lingling Zhang, Eviatar Nevo, Junhua Peng

Affiliations

  1. Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China ; Graduate University of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, People's Republic of China.
  2. Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China.
  3. Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905 Israel.

PMID: 25202562 PMCID: PMC4103125 DOI: 10.3732/apps.1200004

Abstract

PREMISE OF THE STUDY: Tung tree, Vernicia fordii, is native to China. Little has been done on genetics and breeding at the molecular level in this species, let alone utilizing microsatellite (simple sequence repeat [SSR]) markers. Therefore, a set of SSR molecular markers was developed for studies on molecular genetics and breeding in tung tree. •

METHODS AND RESULTS: We designed 78 SSR markers using a protocol based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Assessed in 81 V. fordii accessions, 40 of these markers were polymorphic and 12 of them showed monomorphism. When tested using six V. montana accessions, 52 of the markers were capable of PCR amplification and 25 were polymorphic. •

CONCLUSIONS: The newly developed SSR markers are effective and helpful in the evaluation of genetic germplasm and molecular breeding in tung tree.

Keywords: Euphorbiaceae; FIASCO protocol; Vernicia fordii.; diversity evaluation; microsatellite marker

References

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