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Ng CH, Lee CT, Lee SL, et al. Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae). Appl Plant Sci. 2013;1(7)doi: 10.3732/apps.1200538.
Ng, C. H., Lee, C. T., Lee, S. L., Tnah, L. H., & Ng, K. K. (2013). Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae). Applications in plant sciences, 1(7), . https://doi.org/10.3732/apps.1200538
Ng, Chin Hong, et al. "Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae)." Applications in plant sciences vol. 1,7 (2013). doi: https://doi.org/10.3732/apps.1200538
Ng CH, Lee CT, Lee SL, Tnah LH, Ng KK. Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae). Appl Plant Sci. 2013 Jun 21;1(7). doi: 10.3732/apps.1200538. eCollection 2013 Jul. PMID: 25202566; PMCID: PMC4103129.
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Appl Plant Sci. 2013 Jun 21;1(7). doi: 10.3732/apps.1200538. eCollection 2013 Jul.

Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae).

Applications in plant sciences

Chin Hong Ng, Chai Ting Lee, Soon Leong Lee, Lee Hong Tnah, Kevin Kit Siong Ng

Affiliations

  1. Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.

PMID: 25202566 PMCID: PMC4103129 DOI: 10.3732/apps.1200538

Abstract

PREMISE OF THE STUDY: Microsatellite markers were isolated and characterized in Shorea platyclados (Dipterocarpaceae) for DNA profiling and genetic diversity assessment of this tropical timber species. •

METHODS AND RESULTS: Fifteen polymorphic microsatellite loci were developed and characterized in S. platyclados using a genomic library enriched for dinucleotide (CT) repeats. The primers amplified dinucleotide repeats with 3-14 alleles per locus across four natural populations. The observed and expected heterozygosities ranged from 0.292 to 1.000 and from 0.301 to 0.894, respectively. No significant deviation from Hardy-Weinberg equilibrium was detected in the 15 loci. Four loci pairs displayed linkage disequilibrium. •

CONCLUSIONS: These highly polymorphic markers are adequate for DNA profiling and studies of population genetics in S. platyclados.

Keywords: DNA marker; Shorea platyclados.; hill dipterocarp; microsatellite

References

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  3. Biotechniques. 1994 Apr;16(4):656-60, 662 - PubMed

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