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Hughes PW, Jaworski AF, Davis CS, et al. Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.). Appl Plant Sci. 2014;2(4)doi: 10.3732/apps.1300096.
Hughes, P. W., Jaworski, A. F., Davis, C. S., Aitken, S. M., & Simons, A. M. (2014). Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.). Applications in plant sciences, 2(4), . https://doi.org/10.3732/apps.1300096
Hughes, P William, et al. "Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.)." Applications in plant sciences vol. 2,4 (2014). doi: https://doi.org/10.3732/apps.1300096
Hughes PW, Jaworski AF, Davis CS, Aitken SM, Simons AM. Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.). Appl Plant Sci. 2014 Apr 04;2(4). doi: 10.3732/apps.1300096. eCollection 2014 Apr. PMID: 25202618; PMCID: PMC4103137.
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Appl Plant Sci. 2014 Apr 04;2(4). doi: 10.3732/apps.1300096. eCollection 2014 Apr.

Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.).

Applications in plant sciences

P William Hughes, Allison F Jaworski, Corey S Davis, Susan M Aitken, Andrew M Simons

Affiliations

  1. Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada.
  2. Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Alberta, Canada.

PMID: 25202618 PMCID: PMC4103137 DOI: 10.3732/apps.1300096

Abstract

PREMISE OF THE STUDY: Nuclear microsatellite markers were developed for Lobelia inflata (Campanulaceae), an obligately self-fertilizing plant species, for use in the study of temporal fluctuation in allele frequency and of the genetic structure within and among populations. •

METHODS AND RESULTS: We developed 28 primer pairs for L. inflata, all of which amplify CT dinucleotide repeats. We evaluated amplification of these loci in 53 L. inflata individuals at three sites in eastern North America and found that 24 loci showed microsatellite polymorphism. We also found that 16 loci amplified successfully in L. cardinalis, and 11 amplified successfully in L. siphilitica. •

CONCLUSIONS: These primers will be useful for assessing allelic diversity within and among populations of L. inflata, and show potential for use in congeneric species.

Keywords: Campanulaceae; Lobelia; Lobelia cardinalis; Lobelia inflata; Lobelia siphilitica; microsatellite

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