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Devkota S, Cornejo C, Werth S, et al. Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae). Appl Plant Sci. 2014;2(5)doi: 10.3732/apps.1300101.
Devkota, S., Cornejo, C., Werth, S., Chaudhary, R. P., & Scheidegger, C. (2014). Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae). Applications in plant sciences, 2(5), . https://doi.org/10.3732/apps.1300101
Devkota, Shiva, et al. "Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae)." Applications in plant sciences vol. 2,5 (2014). doi: https://doi.org/10.3732/apps.1300101
Devkota S, Cornejo C, Werth S, Chaudhary RP, Scheidegger C. Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae). Appl Plant Sci. 2014 Apr 05;2(5). doi: 10.3732/apps.1300101. eCollection 2014 May. PMID: 25202623; PMCID: PMC4103109.
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Appl Plant Sci. 2014 Apr 05;2(5). doi: 10.3732/apps.1300101. eCollection 2014 May.

Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae).

Applications in plant sciences

Shiva Devkota, Carolina Cornejo, Silke Werth, Ram Prasad Chaudhary, Christoph Scheidegger

Affiliations

  1. Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland ; Central Department of Botany, Tribhuvan University, Kirtipur, Nepal.
  2. Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland.
  3. Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland ; Faculty of Life and Environmental Sciences, University of Iceland, Reykjavik, Iceland.
  4. Central Department of Botany, Tribhuvan University, Kirtipur, Nepal ; Research Centre for Applied Science and Technology (RECAST), Tribhuvan University, Kirtipur, Nepal.

PMID: 25202623 PMCID: PMC4103109 DOI: 10.3732/apps.1300101

Abstract

PREMISE OF THE STUDY: Microsatellite loci were developed for the rare, Himalayan, endemic haploid lichen fungus, Lobaria pindarensis, to study its population subdivision and the species' response to forest disturbance and fragmentation. •

METHODS AND RESULTS: We developed 18 polymorphic microsatellite markers using 454 pyrosequencing data and assessed them in 109 individuals. The number of alleles per locus ranged from three to 11 with an average of 6.9. Nei's unbiased gene diversity, averaged over loci, ranged from 0.514 to 0.685 in the three populations studied. The cross-amplification success with related species (L. chinensis, L. gyrophorica, L. isidiophora, L. orientalis, L. pulmonaria, L. spathulata, and Lobaria sp.) was generally high and decreased with decreasing relationship to L. pindarensis. •

CONCLUSIONS: The new markers will allow the study of genetic diversity and differentiation within L. pindarensis across its distribution. Moreover, they will enable us to study the effects of forest management on the genetic population structure of this tree-colonizing lichen and to carry out population genetic studies of related species in East Asia.

Keywords: Ascomycetes; Himalayas; Lobaria pindarensis; lichen-forming fungi; microsatellites; population subdivision

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