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Zölzer F, Mußfeldt T, Streffer C. Differential S-phase progression after irradiation of p53 functional versus non-functional tumour cells. Radiol Oncol. 2014;48(4):354-60doi: 10.2478/raon-2014-0032.
Zölzer, F., Mußfeldt, T., & Streffer, C. (2014). Differential S-phase progression after irradiation of p53 functional versus non-functional tumour cells. Radiology and oncology, 48(4), 354-60. https://doi.org/10.2478/raon-2014-0032
Zölzer, Friedo, et al. "Differential S-phase progression after irradiation of p53 functional versus non-functional tumour cells." Radiology and oncology vol. 48,4 (2014): 354-60. doi: https://doi.org/10.2478/raon-2014-0032
Zölzer F, Mußfeldt T, Streffer C. Differential S-phase progression after irradiation of p53 functional versus non-functional tumour cells. Radiol Oncol. 2014 Nov 05;48(4):354-60. doi: 10.2478/raon-2014-0032. eCollection 2014 Dec. PMID: 25435848; PMCID: PMC4230555.
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Radiol Oncol. 2014 Nov 05;48(4):354-60. doi: 10.2478/raon-2014-0032. eCollection 2014 Dec.

Differential S-phase progression after irradiation of p53 functional versus non-functional tumour cells.

Radiology and oncology

Friedo Zölzer, Tamare Mußfeldt, Christian Streffer

Affiliations

  1. Institute of Medical Radiobiology, Medical Faculty, University Duisburg-Essen, Germany ; Department of Radiology, Toxicology and Civil Protection, Faculty of Health and Social Studies, University of South Bohemia in ?eské Bud?jovice, Czech Republic.
  2. Institute of Medical Radiobiology, Medical Faculty, University Duisburg-Essen, Germany.

PMID: 25435848 PMCID: PMC4230555 DOI: 10.2478/raon-2014-0032

Abstract

BACKGROUND: Many pathways seem to be involved in the regulation of the intra-S-phase checkpoint after exposure to ionizing radiation, but the role of p53 has proven to be rather elusive. Here we have a closer look at the progression of irradiated cells through S-phase in dependence of their p53 status.

MATERIALS AND METHODS: Three pairs of tumour cell lines were used, each consisting of one p53 functional and one p53 non-functional line. Cells were labelled with bromodeoxyuridine(BrdU) immediately after irradiation, they were then incubated in label-free medium, and at different times afterwards their position within the S-phase was determined by means of flow cytometry.

RESULTS: While in the p53 deficient cells progression through S-phase was slowed significantly over at least a few hours, it was halted for just about an hour in the p53 proficient cells and then proceeded without further delay or even at a slightly accelerated pace.

CONCLUSIONS: It is clear from the experiments presented here that p53 does play a role for the progress of cells through the S-phase after X-ray exposure, but the exact mechanisms by which replicon initiation and elongation is controlled in irradiated cells remain to be elucidated.

Keywords: flow cytometry, relative movement; intra-S-phase checkpoint; x-rays

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