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Abhari A, Zarghami N, Farzadi L, et al. Altered of microRNA expression level in oligospermic patients. Iran J Reprod Med. 2014;12(10):681-6
Abhari, A., Zarghami, N., Farzadi, L., Nouri, M., & Shahnazi, V. (2014). Altered of microRNA expression level in oligospermic patients. Iranian journal of reproductive medicine, 12(10), 681-6.
Abhari, Alireza, et al. "Altered of microRNA expression level in oligospermic patients." Iranian journal of reproductive medicine vol. 12,10 (2014): 681-6.
Abhari A, Zarghami N, Farzadi L, Nouri M, Shahnazi V. Altered of microRNA expression level in oligospermic patients. Iran J Reprod Med. 2014 Oct;12(10):681-6. PMID: 25469126; PMCID: PMC4248154.
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Iran J Reprod Med. 2014 Oct;12(10):681-6.

Altered of microRNA expression level in oligospermic patients.

Iranian journal of reproductive medicine

Alireza Abhari, Nosratollah Zarghami, Laya Farzadi, Mohammad Nouri, Vahideh Shahnazi

Affiliations

  1. Department. of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  2. Women's Reproductive Health Research Center, Al-zahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran .

PMID: 25469126 PMCID: PMC4248154

Abstract

BACKGROUND: MicroRNA (miRNA) is small endogenous, single strand RNA molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. Mutation and/or aberrant expression of miRNAs have been associated with progression of various disorders, including infertility.

OBJECTIVE: The purpose of this research was to study the estrogen receptor beta (ERβ(, hsa-mir-21 and, hsa-mir-22 expression level in oligospermic infertile and control fertile men and correlation between them.

MATERIALS AND METHODS: In this study, the change in mir-21, mir-22 expression and their common target gene (ERβ) expression levels were evaluated in oligospermic infertile men (n= 43) compared with 43 age matched healthy control by Real-Time PCR methods.

RESULTS: Expression analysis by qRT-PCR test on miRNA have identified that mir-21, mir-22 levels were significantly higher than those in normal controls (p<0.0001) and ERβ expression level significantly decreased in comparison with the normal group (p<0.0001).

CONCLUSION: Our study showed that mir-21 and mir-22 are indirectly involved in spermatogenesis by regulating of the estrogen receptor and might have a diagnostic and prognostic value in men infertility.

Keywords: Fertility; Oligospermia; microRNA; mir-21; mir-22

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