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van Gestel MA, Sanders LE, de Jong JW, et al. FTO knockdown in rat ventromedial hypothalamus does not affect energy balance. Physiol Rep. 2014;2(12)doi: 10.14814/phy2.12152.
van Gestel, M. A., Sanders, L. E., de Jong, J. W., Luijendijk, M. C., & Adan, R. A. (2014). FTO knockdown in rat ventromedial hypothalamus does not affect energy balance. Physiological reports, 2(12), . https://doi.org/10.14814/phy2.12152
van Gestel, Margriet A, et al. "FTO knockdown in rat ventromedial hypothalamus does not affect energy balance." Physiological reports vol. 2,12 (2014). doi: https://doi.org/10.14814/phy2.12152
van Gestel MA, Sanders LE, de Jong JW, Luijendijk MC, Adan RA. FTO knockdown in rat ventromedial hypothalamus does not affect energy balance. Physiol Rep. 2014 Dec 11;2(12). doi: 10.14814/phy2.12152. Print 2014 Dec 01. PMID: 25501432; PMCID: PMC4332191.
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Physiol Rep. 2014 Dec 11;2(12). doi: 10.14814/phy2.12152. Print 2014 Dec 01.

FTO knockdown in rat ventromedial hypothalamus does not affect energy balance.

Physiological reports

Margriet A van Gestel, Loek E Sanders, Johannes W de Jong, Mieneke C M Luijendijk, Roger A H Adan

Affiliations

  1. Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht, Utrecht, The Netherlands.

PMID: 25501432 PMCID: PMC4332191 DOI: 10.14814/phy2.12152

Abstract

Single nucleotide polymorphisms (SNPs) clustered in the first intron of the fat mass and obesity-associated (FTO) gene has been associated with obesity. FTO expression is ubiquitous, with particularly high levels in the hypothalamic area of the brain. To investigate the region-specific role of FTO, AAV technology was applied to knockdown FTO in the ventromedial hypothalamus (VMH). No effect of FTO knockdown was observed on bodyweight or parameters of energy balance. Animals were exposed twice to an overnight fast, followed by a high-fat high-sucrose (HFHS) diet for 1 week. FTO knockdown did not result in a different response to the diets. A region-specific role for FTO in the VMH in the regulation of energy balance could not be found.

© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Keywords: FTO; Feeding; obesity; ventromedial hypothalamus

References

  1. Hum Gene Ther. 2010 Jun;21(6):673-82 - PubMed
  2. PLoS One. 2010 Jan 19;5(1):e8771 - PubMed
  3. N Engl J Med. 2008 Dec 11;359(24):2558-66 - PubMed
  4. Arch Gen Psychiatry. 2010 Mar;67(3):220-9 - PubMed
  5. Cell Mol Life Sci. 2012 Sep;69(18):3127-45 - PubMed
  6. Nat Genet. 2007 Jun;39(6):724-6 - PubMed
  7. Obesity (Silver Spring). 2008 Aug;16(8):1961-5 - PubMed
  8. PLoS Genet. 2007 Jul;3(7):e115 - PubMed
  9. Obes Res. 2005 May;13(5):945-51 - PubMed
  10. PLoS Genet. 2009 Aug;5(8):e1000599 - PubMed
  11. Am J Clin Nutr. 2009 Dec;90(6):1483-8 - PubMed
  12. PLoS One. 2011;6(11):e27968 - PubMed
  13. Prev Med. 1987 Mar;16(2):235-51 - PubMed
  14. Science. 2007 May 11;316(5826):889-94 - PubMed
  15. Learn Mem. 2011 Oct 03;18(10):678-87 - PubMed
  16. FEBS J. 2006 Dec;273(23):5421-7 - PubMed
  17. J Clin Endocrinol Metab. 2009 Apr;94(4):1281-7 - PubMed
  18. Nature. 2009 Apr 16;458(7240):894-8 - PubMed
  19. J Clin Endocrinol Metab. 2008 Jul;93(7):2904-8 - PubMed
  20. Am J Clin Nutr. 2008 Oct;88(4):971-8 - PubMed
  21. Gene Ther. 2014 Feb;21(2):205-11 - PubMed
  22. Front Mol Neurosci. 2011 Jul 04;4:8 - PubMed
  23. Behav Genet. 1997 Jul;27(4):325-51 - PubMed
  24. N Engl J Med. 1990 May 24;322(21):1530 - PubMed
  25. Yale J Biol Med. 1943 Jul;15(6):831-53 - PubMed
  26. Int J Obes (Lond). 2009 Jan;33(1):42-5 - PubMed
  27. N Engl J Med. 1995 Sep 14;333(11):677-85 - PubMed
  28. N Engl J Med. 1986 Jan 23;314(4):193-8 - PubMed
  29. BMC Med Genet. 2010 Apr 09;11:57 - PubMed
  30. Gastroenterology. 2007 May;132(6):2087-102 - PubMed
  31. PLoS Genet. 2013;9(1):e1003166 - PubMed
  32. Cell Metab. 2014 May 6;19(5):767-79 - PubMed
  33. Nature. 2014 Mar 20;507(7492):371-5 - PubMed
  34. FEBS Lett. 2008 Oct 15;582(23-24):3313-9 - PubMed
  35. Horm Behav. 1977 Feb;8(1):40-51 - PubMed
  36. Obesity (Silver Spring). 2012 Dec;20(12 ):2420-5 - PubMed
  37. Science. 2007 Nov 30;318(5855):1469-72 - PubMed
  38. JAMA. 1986 Jul 4;256(1):51-4 - PubMed
  39. Lancet. 2011 Feb 12;377(9765):557-67 - PubMed
  40. Arch Intern Med. 2001 Jul 9;161(13):1581-6 - PubMed
  41. PLoS One. 2010 Nov 16;5(11):e14005 - PubMed
  42. Nat Genet. 2010 Dec;42(12 ):1086-92 - PubMed
  43. Exp Clin Endocrinol Diabetes. 2009 Apr;117(4):194-7 - PubMed
  44. Endocrinology. 1997 Mar;138(3):947-54 - PubMed
  45. Diabetes. 2008 Apr;57(4):1147-50 - PubMed
  46. Int J Obes (Lond). 2009 Jun;33(6):669-79 - PubMed

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