Display options
Share it on

Psychiatr Danub. 2014 Dec;26:546-51.

Melitococcosis in the republic of croatia.

Psychiatria Danubina

Željko Cvetnić, Silvio Špičić, Tomislav Kiš, Maja Zdelar-Tuk, Sanja Duvnjak, Ivana Račić, Miroslav Benić, Boris Habrun, Irena Reil, Zvonimir Šostar

Affiliations

  1. Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Savska cesta 143, 10000 Zagreb, Croatia, [email protected] Croatia.

PMID: 25536995

Abstract

BACKGROUND: Melitococcosis is one of the most widespread zoonoses worldwide. In the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the Republic of Croatia.

METHODS AND RESULTS: During the testing, the Rose Bengal test was applied to 344019 blood samples of sheep and goats, and positive reactions were confirmed in 1143 (0.3%) of samples. The complement fixation test (confirmatory test) was conducted on 43428 samples, with positive reactions confirmed in 768 (1.8%) of samples. The organs and tissues of 336 sheep and goats were inspected bacteriologically, and Brucella sp. was isolated in 15 (4.5%) of samples. Positive serological and bacteriological reactions were confirmed in the Karlovac, Lika-Senj and Split-Dalmatia Counties. Bacteriological and molecular techniques (Bru-up/Bru-low and Bruce-Ladder) in isolates proved the presence of Brucella melitensis biovar 3.

CONCLUSION: On the basis of this study, it can be concluded that Croatia has a favourable situation concerning the infection of ruminants with B. melitensis, and that ongoing controls of the disease are necessary.

Publication Types