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Chen Y, Yang S, Ho EA. Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations. Chromatographia. 2014;77(23):1713-1720doi: 10.1007/s10337-014-2777-7.
Chen, Y., Yang, S., & Ho, E. A. (2014). Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations. Chromatographia, 77(23), 1713-1720. https://doi.org/10.1007/s10337-014-2777-7
Chen, Yufei, et al. "Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations." Chromatographia vol. 77,23 (2014): 1713-1720. doi: https://doi.org/10.1007/s10337-014-2777-7
Chen Y, Yang S, Ho EA. Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations. Chromatographia. 2014;77(23):1713-1720. doi: 10.1007/s10337-014-2777-7. Epub 2014 Oct 17. PMID: 25477555; PMCID: PMC4244548.
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Chromatographia. 2014;77(23):1713-1720. doi: 10.1007/s10337-014-2777-7. Epub 2014 Oct 17.

Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations.

Chromatographia

Yufei Chen, Sidi Yang, Emmanuel A Ho

Affiliations

  1. Laboratory for Drug Delivery and Biomaterials, Faculty of Pharmacy, University of Manitoba, Apotex Centre, 750 McDermot Ave, Room 329, Winnipeg, MB R3E 0T5 Canada.

PMID: 25477555 PMCID: PMC4244548 DOI: 10.1007/s10337-014-2777-7

Abstract

Recently, a growing number of macromolecules such as peptides and proteins have been formulated into various microbicide formulations for the prevention of sexually transmitted infections. However, a fast and reliable high-throughput method for quantitating peptide/protein in polymer-based microbicide formulations is still lacking. As a result, we developed and validated a reversed-phase high-performance liquid chromatography method for the quantitation of gp120 fragment and LL-37 simultaneously in various microbicide gel formulations. This method was capable of detecting a limit of linearity (regression coefficient of 0.999) for gp120 fragment and LL-37 within a range of 0.625-80 and 1.25-80 µg mL

Keywords: Intravaginal gel; Protein degradant; Reversed-phase HPLC; Separation; Tris-tricine SDS-PAGE

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