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Hubacek JA, Lanska V, Adamkova V. Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study. Genet Mol Biol. 2014;37(4):625-30doi: 10.1590/S1415-47572014005000023.
Hubacek, J. A., Lanska, V., & Adamkova, V. (2014). Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study. Genetics and molecular biology, 37(4), 625-30. https://doi.org/10.1590/S1415-47572014005000023
Hubacek, Jaroslav A, et al. "Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study." Genetics and molecular biology vol. 37,4 (2014): 625-30. doi: https://doi.org/10.1590/S1415-47572014005000023
Hubacek JA, Lanska V, Adamkova V. Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study. Genet Mol Biol. 2014 Oct;37(4):625-30. doi: 10.1590/S1415-47572014005000023. Epub 2014 Nov 14. PMID: 25505836; PMCID: PMC4261961.
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Genet Mol Biol. 2014 Oct;37(4):625-30. doi: 10.1590/S1415-47572014005000023. Epub 2014 Nov 14.

Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study.

Genetics and molecular biology

Jaroslav A Hubacek, Vera Lanska, Vera Adamkova

Affiliations

  1. Center for Experimental Medicine , Institute for Clinical and Experimental Medicine , Prague , Czech Republic .
  2. Statistical Unit , Institute for Clinical and Experimental Medicine , Prague , Czech Republic .
  3. Department of Preventive Cardiology , Institute for Clinical and Experimental Medicine , Prague , Czech Republic .

PMID: 25505836 PMCID: PMC4261961 DOI: 10.1590/S1415-47572014005000023

Abstract

Smoking has a significant heritable component of approximately 30-60%. Recent genome wide association studies have identified single nucleotide polymorphisms (SNPs) within the nicotinic cholinergic receptor subunits 3 (rs578776), 5 (rs16969968) and β3 (rs6474412), which are associated with nicotine dependence in Western European populations. To analyze the association in a Czech population, we genotyped 1,191 males and 1,368 females (post-MONICA study). The WHO protocol was used to examine smoking status and the number of cigarettes smoked per day. There were 32.1% current and 27.6% past smokers among the males and 22.5% current and 13.8% past smokers among the females. We have not confirmed the original results: the SNPs rs16969968 (p = 0.07), rs578776 (p = 0.16) and rs6474412 (p = 0.76) were not associated with smoking status (never-smokers vs. ever-smokers) in the entire population, if a codominant model of analysis was used. This result was valid for both the male and female subpopulations if analyzed separately and adjusted for age. Finally, in ever-smokers, the number of cigarettes smoked per day was also independent of different genotypes, regardless of which polymorphism (and gender) was analyzed (the lowest p value was 0.49). The association between the cholinergic receptors-nicotinic subunits (-3, -5 and -ß3), and smoking behavior may be population-dependent.

Keywords: cholinergic receptors; polymorphism; smoking

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