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Soleimanpour S, Hamedi Asl D, Tadayon K, et al. Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberc Res Treat. 2014;2014:195287doi: 10.1155/2014/195287.
Soleimanpour, S., Hamedi Asl, D., Tadayon, K., Farazi, A. A., Keshavarz, R., Soleymani, K., Seddighinia, F. S., & Mosavari, N. (2014). Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberculosis research and treatment, 2014195287. https://doi.org/10.1155/2014/195287
Soleimanpour, Saman, et al. "Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran." Tuberculosis research and treatment vol. 2014 (2014): 195287. doi: https://doi.org/10.1155/2014/195287
Soleimanpour S, Hamedi Asl D, Tadayon K, Farazi AA, Keshavarz R, Soleymani K, Seddighinia FS, Mosavari N. Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran. Tuberc Res Treat. 2014;2014:195287. doi: 10.1155/2014/195287. Epub 2014 Nov 19. PMID: 25505987; PMCID: PMC4253705.
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Tuberc Res Treat. 2014;2014:195287. doi: 10.1155/2014/195287. Epub 2014 Nov 19.

Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran.

Tuberculosis research and treatment

Saman Soleimanpour, Daryoush Hamedi Asl, Keyvan Tadayon, Ali Asghar Farazi, Rouhollah Keshavarz, Kioomars Soleymani, Fereshteh Sadat Seddighinia, Nader Mosavari

Affiliations

  1. PPD Tuberculin Department, Razi Vaccine & Serum Research Institute, Karaj 3197619751, Iran ; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  2. PPD Tuberculin Department, Razi Vaccine & Serum Research Institute, Karaj 3197619751, Iran.
  3. PPD Tuberculin Department, Razi Vaccine & Serum Research Institute, Karaj 3197619751, Iran ; Aerobic Bacterial Research and Vaccine Production Department, Razi Vaccine & Serum Research Institute, Karaj, Iran.
  4. Arak University of Medical Sciences, Arak, Iran.

PMID: 25505987 PMCID: PMC4253705 DOI: 10.1155/2014/195287

Abstract

Human tuberculosis caused by Mycobacterium tuberculosis (Mtb) remains a significant disease in many countries. According to Iran's borders with Afghanistan and Pakistan, which are among the 22 high burden countries around the world, this study was conducted to analyze the current molecular epidemiology of tuberculosis and survey genetic diversity of Mtb strains in Markazi Province in center of Iran. In this experimental study, 75 sputum specimens and one gastric lavage from all smear-positive TB patients admitted to the public hospitals across the Markazi Province were cultured on specific mycobacterial culture media. Genomic DNA was digested by PvuII and transferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS and DR probes. Genotyping of the isolates by PGRS-RFLP and DR-RFLP displayed a wide range of genetic diversity as 25 and 26 genotypes were identified, respectively. Generally speaking, despite the relatively limited number of isolates in the study, high age of patients and also large heterogeneity found in the setting are both in opposition to active circulation of Mtb strains between patients under study either Iranian or Afghan nationals. Thus, it seems that reactivation of latent infection has had the main role in the spread of tuberculosis.

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