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Tamizh Selvan G, Chaudhury NK, Venkatachalam P. Comparison of results of the manual and automated scoring of micronucleus frequencies in (60)Co-irradiated peripheral blood lymphocytes for triage dosimetry. Appl Radiat Isot. 2014;97:70-77doi: 10.1016/j.apradiso.2014.12.018.
Tamizh Selvan, G., Chaudhury, N. K., & Venkatachalam, P. (2015). Comparison of results of the manual and automated scoring of micronucleus frequencies in (60)Co-irradiated peripheral blood lymphocytes for triage dosimetry. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 9770-77. https://doi.org/10.1016/j.apradiso.2014.12.018
Tamizh Selvan, G, et al. "Comparison of results of the manual and automated scoring of micronucleus frequencies in (60)Co-irradiated peripheral blood lymphocytes for triage dosimetry." Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine vol. 97 (2015): 70-77. doi: https://doi.org/10.1016/j.apradiso.2014.12.018
Tamizh Selvan G, Chaudhury NK, Venkatachalam P. Comparison of results of the manual and automated scoring of micronucleus frequencies in (60)Co-irradiated peripheral blood lymphocytes for triage dosimetry. Appl Radiat Isot. 2015 Mar;97:70-77. doi: 10.1016/j.apradiso.2014.12.018. Epub 2014 Dec 19. PMID: 25544665.
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Appl Radiat Isot. 2015 Mar;97:70-77. doi: 10.1016/j.apradiso.2014.12.018. Epub 2014 Dec 19.

Comparison of results of the manual and automated scoring of micronucleus frequencies in (60)Co-irradiated peripheral blood lymphocytes for triage dosimetry.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

G Tamizh Selvan, N K Chaudhury, P Venkatachalam

Affiliations

  1. Sri Ramachandra University, Department of Human Genetics, Porur, Chennai 600 116, Tamil Nadu, India; Institute of Nuclear Medicine and Allied Sciences, Timarpur, New Delhi 110 054, India.
  2. Institute of Nuclear Medicine and Allied Sciences, Timarpur, New Delhi 110 054, India.
  3. Sri Ramachandra University, Department of Human Genetics, Porur, Chennai 600 116, Tamil Nadu, India. Electronic address: [email protected].

PMID: 25544665 DOI: 10.1016/j.apradiso.2014.12.018

Abstract

Scoring micronuclei in the peripheral blood lymphocytes of individuals exposed to ionizing radiation is a rapid biodosimetry assay. Peripheral blood lymphocytes from five individuals were exposed in vitro to 0-5Gy of (60)Co γ-radiation at a dose rate of 0.76Gy/min. The blood cultures were initiated with RPMI-1640 (80%) supplemented with FBS (20%), stimulated with mitogen and incubated at 37°C for 44h. At the 44th hour, cytochalasin-B (6µg/mL) was added, and the cultures were incubated for 28h more. The cells were harvested with a pre-chilled hypotonic solution (0.075M) and fixed with a Carnoy's solution (methanol/acetic acid 5:1). Giemsa- and propidium-iodide-stained cells affixed to slides for microscopy were scored manually and automatically with the micronucleus scoring software from MetaSystems. The micronucleus frequencies determined in the Giemsa-stained cells by manual and automated scoring were 23.6% different (P<0.0001) with an efficiency of 24.9%. Slides stained with propidium iodide are a better choice for automated scoring than Giemsa-stained ones.

Copyright © 2014 Elsevier Ltd. All rights reserved.

Keywords: Automation; Biodosimetry; Blood; Micronuclei

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