Display options
Cite
Nair DB, Jarrell KF. Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis. Life (Basel). 2015;5(1):85-101doi: 10.3390/life5010085.
Nair, D. B., & Jarrell, K. F. (2015). Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis. Life (Basel, Switzerland), 5(1), 85-101. https://doi.org/10.3390/life5010085
Nair, Divya B, and Jarrell, Ken F. "Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis." Life (Basel, Switzerland) vol. 5,1 (2015): 85-101. doi: https://doi.org/10.3390/life5010085
Nair DB, Jarrell KF. Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis. Life (Basel). 2015 Jan 05;5(1):85-101. doi: 10.3390/life5010085. PMID: 25569238; PMCID: PMC4390842.
Copy Download .nbib Format: NLM
Share it on

Life (Basel). 2015 Jan 05;5(1):85-101. doi: 10.3390/life5010085.

Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis.

Life (Basel, Switzerland)

Divya B Nair, Ken F Jarrell

Affiliations

  1. Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada. [email protected].
  2. Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada. [email protected].

PMID: 25569238 PMCID: PMC4390842 DOI: 10.3390/life5010085

Abstract

Methanococcus maripaludis has two different surface appendages: type IV-like pili and archaella. Both structures are believed to be assembled using a bacterial type IV pilus mechanism. Each structure is composed of multiple subunits, either pilins or archaellins. Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. This enzyme is FlaK for archaellins and EppA for pilins. In addition, both pilins and archaellins are modified with N-linked glycans. The archaellins possess an N-linked tetrasaccharide while the pilins have a pentasaccharide which consists of the archaellin tetrasaccharide but with an additional sugar, an unidentified hexose, attached to the linking sugar. In this report, we show that archaellins can be processed by FlaK in the absence of N-glycosylation and N-glycosylation can occur on archaellins that still retain their signal peptides. In contrast, pilins are not glycosylated unless they have been acted on by EppA to have the signal peptide removed. However, EppA can still remove signal peptides from non-glycosylated pilins. These findings indicate that there is a difference in the order of the posttranslational modifications of pilins and archaellins even though both are type IV pilin-like proteins.

References

  1. J Biol Chem. 1985 Dec 5;260(28):15180-5 - PubMed
  2. J Biol Chem. 1987 Jul 15;262(20):9724-9 - PubMed
  3. J Cell Biol. 1987 Oct;105(4):1821-8 - PubMed
  4. J Bacteriol. 2013 Sep;195(17):3808-18 - PubMed
  5. Arch Microbiol. 2014 Mar;196(3):179-91 - PubMed
  6. MBio. 2013 Nov 05;4(6):e00716-13 - PubMed
  7. FEMS Microbiol Rev. 2001 Apr;25(2):147-74 - PubMed
  8. J Biol Chem. 2012 Dec 21;287(52):43322-30 - PubMed
  9. Biochimie. 1988 Nov;70(11):1493-504 - PubMed
  10. J Bacteriol. 2005 Feb;187(3):829-39 - PubMed
  11. J Mol Microbiol Biotechnol. 2004;7(1-2):41-51 - PubMed
  12. Mol Microbiol. 2003 Feb;47(4):929-41 - PubMed
  13. Biochem J. 2011 Jul 1;437(1):43-52 - PubMed
  14. J Bacteriol. 2007 Feb;189(3):772-8 - PubMed
  15. Extremophiles. 2014 Sep;18(5):905-13 - PubMed
  16. Microbiol Rev. 1979 Jun;43(2):260-96 - PubMed
  17. Mol Microbiol. 2001 Aug;41(3):653-63 - PubMed
  18. J Biol Chem. 2006 Jan 13;281(2):1128-36 - PubMed
  19. Mol Microbiol. 2005 Aug;57(4):878-88 - PubMed
  20. Annu Rev Biochem. 1989;58:173-94 - PubMed
  21. Microbiol Mol Biol Rev. 2014 Jun;78(2):304-41 - PubMed
  22. J Bacteriol. 2004 Oct;186(20):6956-69 - PubMed
  23. J Biol Chem. 2005 Apr 29;280(17):16586-93 - PubMed
  24. J Bacteriol. 2012 Sep;194(18):4876-87 - PubMed
  25. J Bacteriol. 2003 Jun;185(11):3416-28 - PubMed
  26. J Bacteriol. 2012 May;194(10):2693-702 - PubMed
  27. J Bacteriol. 2010 Jun;192(12):3093-102 - PubMed
  28. FEMS Microbiol Lett. 2002 Feb 19;208(1):53-9 - PubMed
  29. FEMS Microbiol Rev. 2011 Jul;35(4):577-608 - PubMed
  30. Biochem Soc Trans. 2011 Jan;39(1):64-9 - PubMed
  31. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  32. Genetics. 1999 Aug;152(4):1439-47 - PubMed
  33. Extremophiles. 2009 May;13(3):403-10 - PubMed
  34. FEMS Microbiol Lett. 2011 Jun;319(1):44-50 - PubMed
  35. Mol Microbiol. 2009 May;72(3):633-44 - PubMed
  36. Arch Microbiol. 2001 Apr;175(4):263-9 - PubMed
  37. Biochem Soc Trans. 2013 Feb 1;41(1):432-5 - PubMed
  38. Carbohydr Res. 2009 Mar 31;344(5):648-53 - PubMed
  39. Mol Microbiol. 2003 Nov;50(4):1339-47 - PubMed
  40. Curr Opin Microbiol. 2007 Dec;10(6):588-90 - PubMed
  41. Mol Microbiol. 2012 Jan;83(1):110-24 - PubMed
  42. Biochemistry. 2014 Apr 1;53(12):1899-907 - PubMed
  43. J Bacteriol. 2009 Nov;191(21):6732-40 - PubMed
  44. Curr Opin Microbiol. 2007 Dec;10(6):617-23 - PubMed
  45. J Bacteriol. 2013 Sep;195(18):4094-104 - PubMed
  46. Microbiology. 2007 Feb;153(Pt 2):305-14 - PubMed
  47. Mol Microbiol. 2007 Nov;66(3):596-609 - PubMed
  48. PLoS One. 2013 Dec 30;8(12):e83961 - PubMed
  49. J Bacteriol. 2011 Feb;193(4):804-14 - PubMed
  50. Res Microbiol. 2012 Nov-Dec;163(9-10):630-44 - PubMed
  51. J Bacteriol. 2004 Jun;186(12):4014-8 - PubMed
  52. Mol Microbiol. 2006 Jul;61(1):259-68 - PubMed
  53. Biochim Biophys Acta. 1987 Apr 27;906(1):69-79 - PubMed
  54. Mol Microbiol. 2002 Nov;46(3):879-87 - PubMed
  55. J Biol Chem. 2005 Feb 18;280(7):5236-41 - PubMed
  56. Trends Microbiol. 2012 Jul;20(7):307-12 - PubMed
  57. Mol Microbiol. 1999 Jul;33(1):1-7 - PubMed
  58. J Bacteriol. 2003 Jul;185(13):3918-25 - PubMed
  59. J Bacteriol. 2008 Aug;190(15):5300-7 - PubMed
  60. Life (Basel). 2013 Jan 24;3(1):86-117 - PubMed
  61. J Bacteriol. 2005 Feb;187(3):972-9 - PubMed

Publication Types