Display options
Cite
David MA, Tayebi M. Detection of protein aggregates in brain and cerebrospinal fluid derived from multiple sclerosis patients. Front Neurol. 2014;5:251doi: 10.3389/fneur.2014.00251.
David, M. A., & Tayebi, M. (2014). Detection of protein aggregates in brain and cerebrospinal fluid derived from multiple sclerosis patients. Frontiers in neurology, 5251. https://doi.org/10.3389/fneur.2014.00251
David, Monique Antoinette, and Tayebi, Mourad. "Detection of protein aggregates in brain and cerebrospinal fluid derived from multiple sclerosis patients." Frontiers in neurology vol. 5 (2014): 251. doi: https://doi.org/10.3389/fneur.2014.00251
David MA, Tayebi M. Detection of protein aggregates in brain and cerebrospinal fluid derived from multiple sclerosis patients. Front Neurol. 2014 Dec 02;5:251. doi: 10.3389/fneur.2014.00251. eCollection 2014. PMID: 25520699; PMCID: PMC4252634.
Copy Download .nbib Format: NLM
Share it on

Front Neurol. 2014 Dec 02;5:251. doi: 10.3389/fneur.2014.00251. eCollection 2014.

Detection of protein aggregates in brain and cerebrospinal fluid derived from multiple sclerosis patients.

Frontiers in neurology

Monique Antoinette David, Mourad Tayebi

Affiliations

  1. Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School , Houston, TX , USA ; Antibody Discovery Laboratory, PrioCam , Houston, TX , USA.
  2. Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School , Houston, TX , USA ; Department of Pathology and Infectious Diseases, Faculty of Health and Medical Sciences, University of Surrey , Guildford , UK.

PMID: 25520699 PMCID: PMC4252634 DOI: 10.3389/fneur.2014.00251

Abstract

Studies of the properties of soluble oligomer species of amyloidogenic proteins, derived from different proteins with little sequence homology, have indicated that they share a common structure and may share similar pathogenic mechanisms. Amyloid β, tau protein, as well as amyloid precursor protein normally associated with Alzheimer's disease and Parkinson's disease were found in lesions and plaques of multiple sclerosis patients. The objective of the study is to investigate whether brain and cerebrospinal fluid (CSF) samples derived from multiple sclerosis patients demonstrate the presence of soluble oligomers normally associated with protein-misfolding diseases such as Alzheimer's disease. We have used anti-oligomer monoclonal antibodies to immunodetect soluble oligomers in CSF and brain tissues derived from multiple sclerosis patients. In this report, we describe the presence of soluble oligomers in the brain tissue and cerebral spinal fluid of multiple sclerosis patients detected with our monoclonal anti-oligomer antibodies with Western blot and Sandwich enzyme-linked immunosorbent assay (sELISA). These results might suggest that protein aggregation plays a role in multiple sclerosis pathogenesis although further and more refined studies are needed to confirm the role of soluble aggregates in multiple sclerosis.

Keywords: monoclonal antibodies; multiple sclerosis; protein-misfolding disease; soluble oligomers

References

  1. Expert Rev Anti Infect Ther. 2007 Aug;5(4):631-8 - PubMed
  2. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4501-6 - PubMed
  3. Arthritis Rheum. 2009 Aug;60(8):2284-93 - PubMed
  4. J Neuroimmunol. 2014 Jul 15;272(1-2):76-85 - PubMed
  5. J Neurochem. 2012 May;121(3):486-94 - PubMed
  6. Science. 2003 Apr 18;300(5618):486-9 - PubMed
  7. EMBO J. 1992 Apr;11(4):1593-7 - PubMed
  8. Annu Rev Immunol. 1997;15:235-70 - PubMed
  9. Sci Am. 1991 Nov;265(5):68-71, 74-6, 78 - PubMed
  10. J Neurochem. 2011 Apr;117(1):143-53 - PubMed
  11. J Neuroimmunol. 2006 Nov;180(1-2):17-28 - PubMed
  12. J Neurol Neurosurg Psychiatry. 2009 Mar;80(3):292-6 - PubMed
  13. Immunol Rev. 2000 Aug;176:171-80 - PubMed
  14. ASN Neuro. 2013;5(1):e00111 - PubMed
  15. Glia. 1995 Oct;15(2):141-51 - PubMed
  16. J Neurochem. 2010 Dec;115(6):1556-67 - PubMed
  17. PLoS One. 2011;6(5):e19998 - PubMed
  18. Ann Neurol. 2009 Jun;65(6):639-49 - PubMed
  19. Autoimmun Rev. 2005 Nov;4(8):549-54 - PubMed

Publication Types