World J Clin Oncol. 2014 Dec 10;5(5):1078-87. doi: 10.5306/wjco.v5.i5.1078.
Blood classical monocytes phenotype is not altered in primary non-small cell lung cancer.
World journal of clinical oncology
Saleh A Almatroodi, Christine F McDonald, Allison L Collins, Ian A Darby, Dodie S Pouniotis
Affiliations
Affiliations
- Saleh A Almatroodi, Applied Medical Sciences College, Qassim University, Buraidah, Saudi Arabia.
PMID: 25493244
PMCID: PMC4259935 DOI: 10.5306/wjco.v5.i5.1078
Abstract
AIM: To evaluate the M1 and M2 monocyte phenotype in patients with non-small cell lung cancer (NSCLC) compared to controls. Also, to examine the expression of Th1 and Th2 cytokines in plasma of NSCLC vs controls.
METHODS: Freshly prepared peripheral blood mononuclear cells samples were obtained from patients with NSCLC (lung adenocarcinoma and squamous cell lung carcinoma) and from non-cancer controls. Flow cytometry was performed to investigate M1 and M2 phenotypes in peripheral monocytes (classical monocytes CD14+, CD45+ and CD16-) using conventional surface markers. Th1 and Th2 cytokine production was also analysed in the plasma using cytometric bead array technique.
RESULTS: There were no significant difference in expression of M1 (HLA-DR) and/or M2 markers (CD163 and CD36) markers on classical monocytes in patients with NSCLC compared to non-cancer controls. Expression of CD11b, CD11c, CD71 and CD44 was also shown to be similar in patients with NSCLC compared to non-cancer controls. Th1 and Th2 cytokines [interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12 (p70), tumor necrosis factor (TNF)-α, TNF-β, and interferon-γ] analysis revealed no significant difference between patients with NSCLC and non-cancer controls.
CONCLUSION: This study shows no alteration in peripheral monocyte phenotype in circulating classical monocytes in patients with NSCLC compared to non-cancer controls. No difference in Th1 and Th2 cytokine levels were noted in the plasma of these patients.
Keywords: Lung cancer; Monocyte; Phenotype; Polarisation; Tumour progression; Tumour regression
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