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J Agric Food Chem. 2015 Feb 04;63(4):1261-1267. doi: 10.1021/jf504863u. Epub 2015 Jan 27.

Light Intensity Regulates LC-PUFA Incorporation into Lipids of Pavlova lutheri and the Final Desaturase and Elongase Activities Involved in Their Biosynthesis.

Journal of agricultural and food chemistry

Freddy Guihéneuf, Virginie Mimouni, Gérard Tremblin, Lionel Ulmann

Affiliations

  1. Botany and Plant Science, School of Natural Sciences, Ryan Institute, National University of Ireland Galway , Galway, County of Galway, Ireland.
  2. PRES LUNAM, Université du Maine, Institut Universitaire Mer et Littoral FR-3473 CNRS, EA 2160 Mer Molécules Santé, UFR Sciences et Techniques, 72085 Le Mans Cedex, France.
  3. IUT Génie Biologique, 53020 Laval Cedex, Pays de la Loire, France.

PMID: 25592433 DOI: 10.1021/jf504863u

Abstract

The microalga Pavlova lutheri is a candidate for the production of omega-3 long-chain polyunsaturated fatty acid (LC-PUFA), due to its ability to accumulate both eicosapentaenoic (EPA) and docosahexaenoic acids. Outstanding questions need to be solved to understand the complexity of n-3 LC-PUFA synthesis and partitioning into lipids, especially its metabolic regulation, and which enzymes and/or abiotic factors control their biosynthesis. In this study, the radioactivity of

Keywords: LC-PUFA incorporation; Pavlova lutheri; in vivo 14C-labeling; light intensity; marine microalga; n-3 LC-PUFA biosynthesis; Δ17-desaturation

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