Display options
Cite
Sanada LS, Tavares MR, Sato KL, et al. Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study. Diabetol Metab Syndr. 2015;7:9doi: 10.1186/s13098-015-0005-8.
Sanada, L. S., Tavares, M. R., Sato, K. L., Ferreira, R. d. a. . S., Neubern, M. C., Castania, J. A., Salgado, H. C., & Fazan, V. P. (2015). Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study. Diabetology & metabolic syndrome, 79. https://doi.org/10.1186/s13098-015-0005-8
Sanada, Luciana Sayuri, et al. "Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study." Diabetology & metabolic syndrome vol. 7 (2015): 9. doi: https://doi.org/10.1186/s13098-015-0005-8
Sanada LS, Tavares MR, Sato KL, Ferreira Rda S, Neubern MC, Castania JA, Salgado HC, Fazan VP. Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study. Diabetol Metab Syndr. 2015 Feb 15;7:9. doi: 10.1186/s13098-015-0005-8. eCollection 2015. PMID: 25717348; PMCID: PMC4339238.
Copy Download .nbib Format: NLM
Share it on

Diabetol Metab Syndr. 2015 Feb 15;7:9. doi: 10.1186/s13098-015-0005-8. eCollection 2015.

Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study.

Diabetology & metabolic syndrome

Luciana Sayuri Sanada, Marcelo Rodrigo Tavares, Karina Laurenti Sato, Renata da Silva Ferreira, Milena Cardoso Maia Neubern, Jaci Ayrton Castania, Helio Cesar Salgado, Valéria Paula Sassoli Fazan

Affiliations

  1. Department of Neuroscience and Behavioral Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP Brazil.
  2. Department of Anatomy, José do Rosário Velano University, Alfenas, MG Brazil.
  3. Departament of Physical Therapy, Federal University of Sergipe, Aracaju, SE Brazil.
  4. Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP Brazil.
  5. Department of Neuroscience and Behavioral Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP Brazil ; Department of Surgery and Anatomy, School of Medicine of Ribeirão Preto, USP, Av. Bandeirantes 3900, Ribeirão Preto, SP Brazil.

PMID: 25717348 PMCID: PMC4339238 DOI: 10.1186/s13098-015-0005-8

Abstract

BACKGROUND: Prospective studies have shown incidence rates of hypertension in diabetes mellitus to be three times that of subjects without diabetes mellitus. The reverse also applies, with the incidence of diabetes two to three times higher in patients with hypertension. Despite this common clinical association, the contribution of each isolated entity in the development of a neuropathy is still not well understood. The aims of the present study were to investigate the presence of peripheral neuropathy in spontaneously hypertensive rats (SHR) and SHR with chronically induced diabetes, using a morphological and morphometric study of the sural nerves.

METHODS: Female SHR and normotensive Wistar rats (WR), 8 weeks old, received a single intravenous injection of streptozotocin (STZ) through the tail vein. Controls from both strains received vehicle. Twelve weeks after the injection, sural nerves were dissected and prepared for light microscopy. Morphometry of sural nerve fascicles and myelinated fibers was performed with the aid of computer software.

RESULTS: The sural nerve myelinated fibers were highly affected by experimental diabetes in normotensive rats, causing mainly the reduction of the fiber size. Hypertensive rats showed characteristics of small fiber neuropathy and a severe reduction of the number and density or Schwann cells. The association between diabetes and hypertension caused an increase on the average size of the myelinated fibers, pointing to a small fiber loss, associated to axonal atrophy.

CONCLUSIONS: Our study gives morphological support to the existence of a neuropathy due to hypertension, which is among one of the most common risk factors for diabetic neuropathy. The association between the two neuropathies showed to be a complex alteration, involving and including both, large and small fibers neuropathy. Hypertension caused, indeed, an exacerbation of the alterations already observed in experimental models of diabetic neuropathy.

Keywords: Experimental diabetes; Experimental hypertension; Morphometry; Myelinated fibers; Spontaneously hypertensive rats; Streptozotocin; Sural nerve

References

  1. J Neurosci Methods. 2008 Feb 15;168(1):8-14 - PubMed
  2. J Auton Nerv Syst. 1999 Sep 24;77(2-3):133-9 - PubMed
  3. Biomed Res Int. 2014;2014:870930 - PubMed
  4. Am J Physiol. 1997 Dec;273(6 Pt 2):H2648-51 - PubMed
  5. Neurosci Lett. 1996 Oct 18;217(2-3):85-8 - PubMed
  6. Neurobiol Dis. 2014 Feb;62:18-30 - PubMed
  7. J Peripher Nerv Syst. 2009 Jun;14(2):137-45 - PubMed
  8. Nat Rev Neurol. 2011 Sep 13;7(10):573-83 - PubMed
  9. Jpn Circ J. 1963 Mar;27:282-93 - PubMed
  10. Tohoku J Exp Med. 1979 Jan;127(1):35-44 - PubMed
  11. J Physiol. 1951 Sep;115(1):101-22 - PubMed
  12. J Anat. 2005 Jan;206(1):93-9 - PubMed
  13. Diabetes. 1977 Jul;26(7):689-92 - PubMed
  14. Diabetologia. 1996 Nov;39(11):1377-84 - PubMed
  15. J Pharmacol Toxicol Methods. 2008 Nov-Dec;58(3):215-21 - PubMed
  16. Circ Res. 1981 Mar;48(3):309-19 - PubMed
  17. Nat Clin Pract Neurol. 2007 Jun;3(6):331-40 - PubMed
  18. Psychophysiology. 2008 Jan;45(1):141-7 - PubMed
  19. Diabetes. 2000 Jun;49(6):1006-15 - PubMed
  20. J Neurol Neurosurg Psychiatry. 1979 Jun;42(6):509-18 - PubMed
  21. J Neurosci Res. 2010 Oct;88(13):2889-98 - PubMed
  22. Ann Neurol. 1982 Feb;11(2):147-54 - PubMed
  23. Eur J Pharmacol. 1999 Jun 30;375(1-3):217-23 - PubMed
  24. Neurosci Lett. 2004 May 20;362(2):131-5 - PubMed
  25. Brain Res. 2008 Jul 30;1222:51-60 - PubMed
  26. Acta Neuropathol. 2012 Oct;124(4):561-73 - PubMed
  27. J Anat. 1971 Sep;109(Pt 3):505-26 - PubMed
  28. Diabetes. 1988 Aug;37(8):1129-36 - PubMed
  29. Brain Res. 2008 Mar 4;1197:170-80 - PubMed
  30. Diabetes. 1990 Jun;39(6):690-6 - PubMed
  31. Brain Res. 2013 Jan 23;1491:197-203 - PubMed
  32. Acta Cir Bras. 2011 Dec;26(6):514-20 - PubMed
  33. Acta Neuropathol. 1981;53(2):99-106 - PubMed
  34. Diabetologia. 1992 Sep;35(9):813-7 - PubMed
  35. J Hum Hypertens. 2009 Nov;23(11):709-17 - PubMed
  36. Braz J Med Biol Res. 2011 Jun;44(6):583-91 - PubMed
  37. J Neurosci Methods. 2005 Oct 30;148(2):130-6 - PubMed
  38. Ann Neurol. 1994 Sep;36(3):408-15 - PubMed
  39. BMC Neurosci. 2012 Mar 02;13:24 - PubMed
  40. J Neurosci Methods. 2006 Mar 15;151(2):131-8 - PubMed
  41. J Comp Neurol. 1988 Mar 8;269(2):203-9 - PubMed
  42. Life Support Syst. 1985;3 Suppl 1:524-9 - PubMed
  43. J Peripher Nerv Syst. 1997;2(3):213-26 - PubMed
  44. J Vet Med Sci. 2012 Dec;74(12):1669-73 - PubMed
  45. Am J Physiol Heart Circ Physiol. 2001 Apr;280(4):H1560-4 - PubMed
  46. J Neurosci Methods. 2007 Mar 30;161(1):17-22 - PubMed
  47. Biomed Res Int. 2013;2013:682849 - PubMed
  48. Acta Neuropathol. 1981;53(4):257-65 - PubMed
  49. J Neuropathol Exp Neurol. 1980 Nov;39(6):710-21 - PubMed
  50. Diabetologia. 1976 Dec;12(6):539-46 - PubMed
  51. Am J Physiol. 1970 Nov;219(5):1256-8 - PubMed
  52. BMC Neurosci. 2014 Jan 05;15:5 - PubMed

Publication Types