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Afara IO, Moody H, Singh S, et al. Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy. Biomed Opt Express. 2014;6(1):144-54doi: 10.1364/BOE.6.000144.
Afara, I. O., Moody, H., Singh, S., Prasadam, I., & Oloyede, A. (2015). Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy. Biomedical optics express, 6(1), 144-54. https://doi.org/10.1364/BOE.6.000144
Afara, Isaac O, et al. "Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy." Biomedical optics express vol. 6,1 (2015): 144-54. doi: https://doi.org/10.1364/BOE.6.000144
Afara IO, Moody H, Singh S, Prasadam I, Oloyede A. Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy. Biomed Opt Express. 2014 Dec 15;6(1):144-54. doi: 10.1364/BOE.6.000144. eCollection 2015 Jan 01. PMID: 25657883; PMCID: PMC4317110.
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Biomed Opt Express. 2014 Dec 15;6(1):144-54. doi: 10.1364/BOE.6.000144. eCollection 2015 Jan 01.

Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy.

Biomedical optics express

Isaac O Afara, Hayley Moody, Sanjleena Singh, Indira Prasadam, Adekunle Oloyede

Affiliations

  1. Department of Applied Physics, University of Eastern Finland, Kuopio, Finland ; School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
  2. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
  3. Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.
  4. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia.

PMID: 25657883 PMCID: PMC4317110 DOI: 10.1364/BOE.6.000144

Abstract

Diagnosis of articular cartilage pathology in the early disease stages using current clinical diagnostic imaging modalities is challenging, particularly because there is often no visible change in the tissue surface and matrix content, such as proteoglycans (PG). In this study, we propose the use of near infrared (NIR) spectroscopy to spatially map PG content in articular cartilage. The relationship between NIR spectra and reference data (PG content) obtained from histology of normal and artificially induced PG-depleted cartilage samples was investigated using principal component (PC) and partial least squares (PLS) regression analyses. Significant correlation was obtained between both data (R(2) = 91.40%, p<0.0001). The resulting correlation was used to predict PG content from spectra acquired from whole joint sample, this was then employed to spatially map this component of cartilage across the intact sample. We conclude that NIR spectroscopy is a feasible tool for evaluating cartilage contents and mapping their distribution across mammalian joint.

Keywords: (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization

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