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Ortega FJ, Bañuls MJ, Sanza FJ, et al. Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors (Basel). 2012;2(3):291-304doi: 10.3390/bios2030291.
Ortega, F. J., Bañuls, M. J., Sanza, F. J., Casquel, R., Laguna, M. F., Holgado, M., López-Romero, D., Barrios, C. A., Maquieira, �. �., & Puchades, R. (2012). Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors, 2(3), 291-304. https://doi.org/10.3390/bios2030291
Ortega, Francisco J, et al. "Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars." Biosensors vol. 2,3 (2012): 291-304. doi: https://doi.org/10.3390/bios2030291
Ortega FJ, Bañuls MJ, Sanza FJ, Casquel R, Laguna MF, Holgado M, López-Romero D, Barrios CA, Maquieira Á, Puchades R. Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors (Basel). 2012 Aug 14;2(3):291-304. doi: 10.3390/bios2030291. PMID: 25585931; PMCID: PMC4263551.
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Biosensors (Basel). 2012 Aug 14;2(3):291-304. doi: 10.3390/bios2030291.

Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars.

Biosensors

Francisco J Ortega, María-José Bañuls, Francisco J Sanza, Rafael Casquel, María Fe Laguna, Miguel Holgado, David López-Romero, Carlos A Barrios, Ángel Maquieira, Rosa Puchades

Affiliations

  1. Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. [email protected].
  2. Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. [email protected].
  3. Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. [email protected].
  4. Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. [email protected].
  5. Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. [email protected].
  6. Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. [email protected].
  7. Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain. [email protected].
  8. Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain. [email protected].
  9. Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. [email protected].
  10. Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. [email protected].

PMID: 25585931 PMCID: PMC4263551 DOI: 10.3390/bios2030291

Abstract

In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).

References

  1. Talanta. 2010 Sep 15;82(4):1581-7 - PubMed
  2. Contraception. 2007 Sep;76(3):221-7 - PubMed
  3. Science. 1997 Oct 31;278(5339):840-3 - PubMed
  4. Biosens Bioelectron. 2008 Mar 14;23(8):1298-302 - PubMed
  5. Anal Biochem. 2010 Jan 1;396(1):30-5 - PubMed
  6. Fertil Steril. 2004 Dec;82(6):1694-6 - PubMed
  7. Opt Lett. 2008 Apr 1;33(7):708-10 - PubMed
  8. Drugs. 2009;69(6):649-75 - PubMed
  9. Biosens Bioelectron. 2006 Jan 15;21(7):1327-32 - PubMed
  10. Anal Biochem. 2009 Feb 15;385(2):234-41 - PubMed
  11. Anal Biochem. 1997 Mar 1;246(1):1-6 - PubMed
  12. Anal Chim Acta. 2008 Jul 14;620(1-2):8-26 - PubMed
  13. Biosens Bioelectron. 2011 Aug 15;26(12):4842-7 - PubMed
  14. Contraception. 1974 Aug;10(2):145-57 - PubMed
  15. Appl Opt. 2001 Nov 1;40(31):5742-7 - PubMed
  16. Opt Lett. 2003 Aug 1;28(15):1329-31 - PubMed
  17. Opt Lett. 1996 Apr 15;21(8):618-20 - PubMed
  18. Langmuir. 2006 Mar 14;22(6):2719-25 - PubMed
  19. Chem Rev. 2008 Feb;108(2):462-93 - PubMed
  20. Anal Chem. 2012 Jan 17;84(2):793-821 - PubMed
  21. Electrophoresis. 1998 Nov;19(16-17):3040-4 - PubMed
  22. Biosens Bioelectron. 2010 Aug 15;25(12):2553-8 - PubMed

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