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Park M, Rafii F. Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains. Int J Microbiol. 2014;2014:456979doi: 10.1155/2014/456979.
Park, M., & Rafii, F. (2014). Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains. International journal of microbiology, 2014456979. https://doi.org/10.1155/2014/456979
Park, Miseon, and Rafii, Fatemeh. "Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains." International journal of microbiology vol. 2014 (2014): 456979. doi: https://doi.org/10.1155/2014/456979
Park M, Rafii F. Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains. Int J Microbiol. 2014;2014:456979. doi: 10.1155/2014/456979. Epub 2014 Dec 21. PMID: 25587280; PMCID: PMC4283427.
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Int J Microbiol. 2014;2014:456979. doi: 10.1155/2014/456979. Epub 2014 Dec 21.

Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains.

International journal of microbiology

Miseon Park, Fatemeh Rafii

Affiliations

  1. Division of Microbiology, National Center for Toxicological Research, U.S. FDA, Jefferson, AR 72079, USA.

PMID: 25587280 PMCID: PMC4283427 DOI: 10.1155/2014/456979

Abstract

Fluoroquinolone resistance affects toxin production of Clostridium perfringens strains differently. To investigate the effect of fluoroquinolone resistance selection on global changes in metabolic activities and drug susceptibilities, four C. perfringens strains and their norfloxacin-, ciprofloxacin-, and gatifloxacin-resistant mutants were compared in nearly 2000 assays, using phenotype microarray plates. Variations among mutant strains resulting from resistance selection were observed in all aspects of metabolism. Carbon utilization, pH range, osmotic tolerance, and chemical sensitivity of resistant strains were affected differently in the resistant mutants depending on both the bacterial genotype and the fluoroquinolone to which the bacterium was resistant. The susceptibilities to gentamicin and erythromycin of all resistant mutants except one increased, but some resistant strains were less susceptible to amoxicillin, cefoxitin, ceftriaxone, chloramphenicol, and metronidazole than their wild types. Sensitivity to ethidium bromide decreased in some resistant mutants and increased in others. Microarray analysis of two gatifloxacin-resistant mutants showed changes in metabolic activities that were correlated with altered expression of various genes. Both the chemical structures of fluoroquinolones and the genomic makeup of the wild types influenced the changes found in resistant mutants, which may explain some inconsistent reports of the effects of therapeutic use of fluoroquinolones on clinical isolates of bacteria.

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