Display options
Cite
Mosier AP, Peters SB, Larsen M, et al. Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy. Biosensors (Basel). 2014;4(1):18-27doi: 10.3390/bios4010018.
Mosier, A. P., Peters, S. B., Larsen, M., & Cady, N. C. (2014). Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy. Biosensors, 4(1), 18-27. https://doi.org/10.3390/bios4010018
Mosier, Aaron P, et al. "Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy." Biosensors vol. 4,1 (2014): 18-27. doi: https://doi.org/10.3390/bios4010018
Mosier AP, Peters SB, Larsen M, Cady NC. Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy. Biosensors (Basel). 2014 Feb 27;4(1):18-27. doi: 10.3390/bios4010018. eCollection 2014 Mar. PMID: 25587408; PMCID: PMC4264367.
Copy Download .nbib Format: NLM
Share it on

Biosensors (Basel). 2014 Feb 27;4(1):18-27. doi: 10.3390/bios4010018. eCollection 2014 Mar.

Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy.

Biosensors

Aaron P Mosier, Sarah B Peters, Melinda Larsen, Nathaniel C Cady

Affiliations

  1. State University of New York (SUNY) College of Nanoscale Science & Engineering, 257 Fuller Rd., Albany, NY 12203, USA; E-Mail: [email protected].
  2. Department of Biological Sciences, State University of New York at Albany, 1400 Washington Avenue, Albany, NY 12222, USA; E-Mails: [email protected] (S.B.P.); [email protected] (M.L.).

PMID: 25587408 PMCID: PMC4264367 DOI: 10.3390/bios4010018

Abstract

The ability to characterize the microscale mechanical properties of biological materials has the potential for great utility in the field of tissue engineering. The development and morphogenesis of mammalian tissues are known to be guided in part by mechanical stimuli received from the local environment, and tissues frequently develop to match the physical characteristics (i.e., elasticity) of their environment. Quantification of these material properties at the microscale may provide valuable information to guide researchers. Presented here is a microfluidic platform for the non-destructive ex vivo microscale mechanical characterization of mammalian tissue samples by atomic force microscopy (AFM). The device was designed to physically hold a tissue sample in a dynamically controllable fluid environment while allowing access by an AFM probe operating in force spectroscopy mode to perform mechanical testing. Results of measurements performed on mouse submandibular gland samples demonstrate the ability of the analysis platform to quantify sample elasticity at the microscale, and observe chemically-induced changes in elasticity.

Keywords: AFM; elasticity; microfluidics; submandibular gland; tissue engineering

References

  1. Dev Biol. 2009 Dec 15;336(2):169-82 - PubMed
  2. J Microbiol Methods. 2012 Oct;91(1):198-204 - PubMed
  3. J Am Dent Assoc. 2003 Jan;134(1):61-9; quiz 118-9 - PubMed
  4. J Biomech. 2010 Jan 5;43(1):55-62 - PubMed
  5. Phys Rev Lett. 1986 Mar 3;56(9):930-933 - PubMed
  6. Methods Cell Biol. 2007;83:347-72 - PubMed
  7. Oral Dis. 2002 Mar;8(2):77-89 - PubMed
  8. Biomaterials. 2011 Oct;32(28):6754-63 - PubMed
  9. Tissue Eng Part A. 2014 Jun;20(11-12):1632-42 - PubMed
  10. Science. 2003 Mar 14;299(5613):1743-7 - PubMed
  11. Curr Protoc Cell Biol. 2008 Dec;Chapter 19:Unit 19.8 - PubMed
  12. Biophys J. 2010 Dec 1;99(11):3639-46 - PubMed
  13. J Biol Chem. 2004 Aug 20;279(34):35557-63 - PubMed

Publication Types

Grant support