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Waaijers S, Ramalho JJ, Koorman T, et al. The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability. Biol Open. 2015;4(3):276-84doi: 10.1242/bio.201410744.
Waaijers, S., Ramalho, J. J., Koorman, T., Kruse, E., & Boxem, M. (2015). The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability. Biology open, 4(3), 276-84. https://doi.org/10.1242/bio.201410744
Waaijers, Selma, et al. "The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability." Biology open vol. 4,3 (2015): 276-84. doi: https://doi.org/10.1242/bio.201410744
Waaijers S, Ramalho JJ, Koorman T, Kruse E, Boxem M. The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability. Biol Open. 2015 Feb 06;4(3):276-84. doi: 10.1242/bio.201410744. PMID: 25661870; PMCID: PMC4359734.
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Biol Open. 2015 Feb 06;4(3):276-84. doi: 10.1242/bio.201410744.

The C. elegans Crumbs family contains a CRB3 homolog and is not essential for viability.

Biology open

Selma Waaijers, João Jacob Ramalho, Thijs Koorman, Elisabeth Kruse, Mike Boxem

Affiliations

  1. Division of Developmental Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
  2. Division of Developmental Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. [email protected].

PMID: 25661870 PMCID: PMC4359734 DOI: 10.1242/bio.201410744

Abstract

Crumbs proteins are important regulators of epithelial polarity. In C. elegans, no essential role for the two described Crumbs homologs has been uncovered. Here, we identify and characterize an additional Crumbs family member in C. elegans, which we termed CRB-3 based on its similarity in size and sequence to mammalian CRB3. We visualized CRB-3 subcellular localization by expressing a translational GFP fusion. CRB-3::GFP was expressed in several polarized tissues in the embryo and larval stages, and showed apical localization in the intestine and pharynx. To identify the function of the Crumbs family in C. elegans development, we generated a triple Crumbs deletion mutant by sequentially removing the entire coding sequence for each crumbs homolog using a CRISPR/Cas9-based approach. Remarkably, animals lacking all three Crumbs homologs are viable and show normal epithelial polarity. Thus, the three C. elegans Crumbs family members do not appear to play an essential role in epithelial polarity establishment.

© 2015. Published by The Company of Biologists Ltd.

Keywords: C. elegans; CRB; Crumbs; cell polarity

References

  1. Eur J Cell Biol. 2008 Mar;87(3):123-36 - PubMed
  2. Cell. 1996 Apr 19;85(2):195-204 - PubMed
  3. Curr Biol. 2010 Apr 13;20(7):582-90 - PubMed
  4. Mol Biol Cell. 2004 Mar;15(3):1324-33 - PubMed
  5. Mol Vis. 2005 Apr 15;11:263-73 - PubMed
  6. Mol Biol Evol. 2013 Apr;30(4):772-80 - PubMed
  7. Curr Biol. 2010 Jun 22;20(12):1065-74 - PubMed
  8. Development. 2010 Jun;137(11):1833-42 - PubMed
  9. Annu Rev Cell Dev Biol. 2012;28:655-85 - PubMed
  10. J Cell Sci. 2004 Aug 15;117(Pt 18):4169-77 - PubMed
  11. Science. 2002 Dec 6;298(5600):1955-9 - PubMed
  12. Genetics. 1974 May;77(1):71-94 - PubMed
  13. Hum Mol Genet. 2006 Sep 15;15(18):2659-72 - PubMed
  14. Dev Biol. 2004 Feb 1;266(1):17-26 - PubMed
  15. Dev Dyn. 2011 Dec;240(12):2646-56 - PubMed
  16. WormBook. 2006 Jun 19;:1-61 - PubMed
  17. Cell. 2010 May 28;141(5):757-74 - PubMed
  18. J Cell Biol. 2007 Jul 30;178(3):387-98 - PubMed
  19. Biotechnology (N Y). 1993 Oct;11(10):1138-43 - PubMed
  20. Biochim Biophys Acta. 2008 Mar;1778(3):614-30 - PubMed
  21. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5 - PubMed
  22. J Cell Sci. 2003 Jul 15;116(Pt 14):2895-906 - PubMed
  23. Curr Biol. 2000 Jan 27;10(2):76-85 - PubMed
  24. Mech Dev. 2002 Jan;110(1-2):203-7 - PubMed
  25. Mol Cell Biol. 2014 Jan;34(1):43-56 - PubMed
  26. Cell. 1998 Sep 18;94(6):761-71 - PubMed
  27. Genome Res. 2011 Feb;21(2):325-41 - PubMed
  28. J Vis Exp. 2008 Aug 15;(18):null - PubMed
  29. Dev Dyn. 2006 Jun;235(6):1501-7 - PubMed
  30. Gene. 2003 Jan 2;302(1-2):21-9 - PubMed
  31. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5786-90 - PubMed
  32. Curr Biol. 2010 Jul 13;20(13):R574-82 - PubMed
  33. Dev Biol. 2006 Oct 15;298(2):379-91 - PubMed
  34. Nucleic Acids Res. 2012 Jan;40(Database issue):D306-12 - PubMed
  35. Hum Mol Genet. 2013 Jan 1;22(1):35-50 - PubMed
  36. J Cell Sci. 2009 Aug 1;122(Pt 15):2587-96 - PubMed
  37. Dev Cell. 2012 May 15;22(5):1101-8 - PubMed
  38. Reprod Domest Anim. 2014 Oct;49(5):824-30 - PubMed
  39. G3 (Bethesda). 2013 Feb;3(2):153-65 - PubMed
  40. Curr Biol. 2010 Apr 13;20(7):573-81 - PubMed
  41. J Cell Biol. 2010 Sep 6;190(5):751-60 - PubMed
  42. Nucleic Acids Res. 2011 Jul;39(Web Server issue):W475-8 - PubMed
  43. PLoS One. 2009;4(3):e4625 - PubMed
  44. Genetics. 2013 Nov;195(3):1187-91 - PubMed
  45. Dev Biol. 2001 Feb 1;230(1):29-42 - PubMed
  46. Development. 2005 Mar;132(5):977-86 - PubMed
  47. Dev Cell. 2006 Sep;11(3):363-74 - PubMed
  48. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10532-7 - PubMed
  49. Genetics. 2000 Feb;154(2):635-46 - PubMed
  50. Eur J Biochem. 1993 May 1;213(3):1333-40 - PubMed
  51. Syst Biol. 2010 May;59(3):307-21 - PubMed
  52. Cell. 1995 Jul 14;82(1):67-76 - PubMed
  53. Cell. 2010 Apr 30;141(3):509-23 - PubMed
  54. Mol Biol Cell. 2009 Nov;20(22):4652-63 - PubMed
  55. J Cell Biol. 2002 Sep 2;158(5):941-51 - PubMed
  56. Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15810-5 - PubMed
  57. Nat Genet. 1999 Oct;23(2):217-21 - PubMed

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