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Puri BK, Segal DR, Monro JA. Diagnostic use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay in confirming active Lyme borreliosis in clinically and serologically ambiguous cases. Int J Clin Exp Med. 2014;7(12):5890-2
Puri, B. K., Segal, D. R., & Monro, J. A. (2014). Diagnostic use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay in confirming active Lyme borreliosis in clinically and serologically ambiguous cases. International journal of clinical and experimental medicine, 7(12), 5890-2.
Puri, Basant K, et al. "Diagnostic use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay in confirming active Lyme borreliosis in clinically and serologically ambiguous cases." International journal of clinical and experimental medicine vol. 7,12 (2014): 5890-2.
Puri BK, Segal DR, Monro JA. Diagnostic use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay in confirming active Lyme borreliosis in clinically and serologically ambiguous cases. Int J Clin Exp Med. 2014 Dec 15;7(12):5890-2. eCollection 2014. PMID: 25664127; PMCID: PMC4307574.
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Int J Clin Exp Med. 2014 Dec 15;7(12):5890-2. eCollection 2014.

Diagnostic use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay in confirming active Lyme borreliosis in clinically and serologically ambiguous cases.

International journal of clinical and experimental medicine

Basant K Puri, Daniel Rm Segal, Jean A Monro

Affiliations

  1. Department of Medicine, Imperial College London, Hammersmith Hospital London W12 0HS, UK.
  2. Breakspear Medical Group Hemel Hempstead, Hertfordshire, HP2 4FD, UK.

PMID: 25664127 PMCID: PMC4307574

Abstract

The aim of this study was to carry out an independent evaluation of the proposition that the lymphocyte transformation test-memory lymphocyte immunostimulation assay (LTT-MELISA) may be diagnostically useful in the confirmation of active Lyme borreliosis in clinically and serologically ambiguous cases. Blood samples from 54 patients consecutively presenting to a British center with clinical suspicion of Lyme borreliosis were tested for this disease by immunoglobulin M (IgM) and immunoglobulin G (IgG) Western blots and by LTT-MELISA. Forty-five of these patients had Western blot results which were negative for both IgM and IgG by the criteria of the Centers for Disease Control and Prevention (CDC); of these patients, 19 (42%) were LTT-MELISA-positive. Two of the patients who had IgM positive results by the CDC criteria were LTT-MELISA-negative. It is concluded that, for putative European-acquired Lyme borreliosis infections, it would be sensible to carry out both the LTT-MELISA and Western blot assay.

Keywords: Lyme borreliosis; MELISA; lymphocyte transformation test

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