Expert Rev Proteomics. 2015 Feb;12(1):61-74. doi: 10.1586/14789450.2015.1002474. Epub 2015 Jan 16.

Proteomics reveals the importance of the dynamic redistribution of the subcellular location of proteins in breast cancer cells.

Expert review of proteomics

Gabriella Pinto, Abdulrab Ahmed M Alhaiek, Jasminka Godovac-Zimmermann

PMID: 25591448 DOI: 10.1586/14789450.2015.1002474

Abstract

At the molecular level, living cells are enormously complicated complex adaptive systems in which intertwined genomic, transcriptomic, proteomic and metabolic networks all play a crucial role. At the same time, cells are spatially heterogeneous systems in which subcellular compartmentalization of different functions is ubiquitous and requires efficient cross-compartmental communication. Dynamic redistribution of multitudinous proteins to different subcellular locations in response to cellular functional state is increasingly recognized as a crucial characteristic of cellular function that seems to be at least as important as overall changes in protein abundance. Characterization of the subcellular spatial dynamics of protein distribution is a major challenge for proteomics and recent results with MCF7 breast cancer cells suggest that this may be of particular importance for cancer cells.

Keywords: MCF7 cells; SILAC; breast cancer; cytoplasm; estrogen receptor; mass spectrometry; nucleus; protein trafficking; quantitative proteomics; subcellular protein location

Substances

• Proteome

MeSH terms

• Humans
• MCF-7 Cells
• Neoplasms / metabolism
• Protein Transport
• Proteome / metabolism
• Proteomics / methods

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't
• Review