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Pandey R, Mehrotra D, Catapano C, et al. Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis. J Oral Biol Craniofac Res. 2012;2(1):4-9doi: 10.1016/S2212-4268(12)60003-7.
Pandey, R., Mehrotra, D., Catapano, C., Choubey, V., Sarin, R., Mahdi, A. A., & Singh, S. (2012). Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis. Journal of oral biology and craniofacial research, 2(1), 4-9. https://doi.org/10.1016/S2212-4268(12)60003-7
Pandey, Rahul, et al. "Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis." Journal of oral biology and craniofacial research vol. 2,1 (2012): 4-9. doi: https://doi.org/10.1016/S2212-4268(12)60003-7
Pandey R, Mehrotra D, Catapano C, Choubey V, Sarin R, Mahdi AA, Singh S. Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis. J Oral Biol Craniofac Res. 2012 Jan-Apr;2(1):4-9. doi: 10.1016/S2212-4268(12)60003-7. PMID: 25756024; PMCID: PMC3941659.
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J Oral Biol Craniofac Res. 2012 Jan-Apr;2(1):4-9. doi: 10.1016/S2212-4268(12)60003-7.

Association of mitochondrial deoxyribonucleic acid mutation with polymorphism in CYP2E1 gene in oral carcinogenesis.

Journal of oral biology and craniofacial research

Rahul Pandey, Divya Mehrotra, Carlo Catapano, Vimal Choubey, Rajiv Sarin, Abbas Ali Mahdi, Stuti Singh

Affiliations

  1. Senior Research Fellow, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, India.
  2. Professor, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, India.
  3. Professor and Director, Institute of Oncology Research, Switzerland.
  4. Research Scholar, Department of Urology, CSM Medical University, Lucknow, India.
  5. Professor and Director, ACTREC, Mumbai, Maharashtra, India.
  6. Professor and Head, Department of Biochemistry, CSM Medical University, Lucknow, India.
  7. Resident, Department of Oral and Maxillofacial Surgery, CSM Medical University, Lucknow, India.

PMID: 25756024 PMCID: PMC3941659 DOI: 10.1016/S2212-4268(12)60003-7

Abstract

BACKGROUND: Oral carcinogenesis is a complex process affected by genetic as well as environmental factors. CYP2E1 gene is involved in metabolism of number of compounds and carcinogens. Its normal functioning is required for homeostasis of free radical. Mitochondrial deoxyribonucleic acid (mtDNA) is 10-100 times more susceptible to damage than nuclear DNA. Mitochondrial DNA large scale deletions are well documented in oral cancer. However, the relationship between CYP2E1 gene polymorphisms and mtDNA damage is still not documented in literature.

MATERIALS AND METHODS: Case-control study involving 50 subjects was carried out. Deoxyribonucleic acid extraction was done from study subject tissue samples. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) amplification was done to confirm CYP2E1 gene polymorphisms. The PCR amplification was done for mtDNA 4977 bp deletion. Statistical analysis was carried out using SPSS version 11.5 with χ(2) tests.

RESULTS: c1c1 and DD polymorphisms are prevalent in North Indian population having oral cancer. These polymorphisms are significantly associated with mtDNA 4977 bp deletion.

CONCLUSION: Mitochondrial DNA damage induced by wild CYP2E1 forms and imperfect DNA repair in mtDNA may act synergistically to greatly enhance oral cancer risk.

Keywords: CYP2E1; DNA; mitochondria; oral cancer

References

  1. Clin Chem. 2002 Nov;48(11):1901-12 - PubMed
  2. Pharmacogenomics. 2008 May;9(5):551-60 - PubMed
  3. Pharmacogenetics. 1994 Apr;4(2):58-63 - PubMed
  4. IARC Sci Publ. 2004;(157):393-414 - PubMed
  5. Jpn J Cancer Res. 1991 Mar;82(3):254-6 - PubMed
  6. Oral Oncol. 2001 Jul;37(5):437-45 - PubMed
  7. Cancer Epidemiol Biomarkers Prev. 1997 Nov;6(11):901-5 - PubMed
  8. J Hepatol. 2004 Nov;41(5):744-50 - PubMed
  9. Oral Oncol. 2006 Feb;42(2):201-7 - PubMed
  10. Science. 2000 Mar 17;287(5460):2017-9 - PubMed
  11. J Natl Cancer Inst. 1997 Aug 20;89(16):1207-12 - PubMed
  12. Cancer Invest. 2002;20(4):557-69 - PubMed
  13. Pharmacogenetics. 1998 Apr;8(2):91-100 - PubMed
  14. J Biochem. 1991 Oct;110(4):559-65 - PubMed
  15. Br J Cancer. 2000 Jun;82(11):1871-4 - PubMed
  16. FEBS Lett. 1998 Dec 18;441(2):292-6 - PubMed
  17. J Oral Pathol Med. 2006 Jan;35(1):11-8 - PubMed
  18. Cancer Epidemiol Biomarkers Prev. 2001 Dec;10(12):1239-48 - PubMed
  19. Cancer Lett. 2003 May 30;195(1):33-42 - PubMed
  20. Exp Oncol. 2005 Dec;27(4):248-56 - PubMed
  21. Chem Res Toxicol. 1991 Mar-Apr;4(2):168-79 - PubMed
  22. Cancer Epidemiol Biomarkers Prev. 2000 Jan;9(1):3-28 - PubMed

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