Front Microbiol. 2015 Jan 23;6:17. doi: 10.3389/fmicb.2015.00017. eCollection 2015.
Polar front associated variation in prokaryotic community structure in Arctic shelf seafloor.
Frontiers in microbiology
Tan T Nguyen, Bjarne Landfald
Affiliations
Affiliations
- Centre for Research-based Innovation on Marine Bioactives and Drug Discovery (MabCent-SFI), UiT The Arctic University of Norway Tromsø, Norway.
- Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT The Arctic University of Norway Tromsø, Norway.
PMID: 25667586
PMCID: PMC4304239 DOI: 10.3389/fmicb.2015.00017
Abstract
Spatial variations in composition of marine microbial communities and its causes have largely been disclosed in studies comprising rather large environmental and spatial differences. In the present study, we explored if a moderate but temporally permanent climatic division within a contiguous arctic shelf seafloor was traceable in the diversity patterns of its bacterial and archaeal communities. Soft bottom sediment samples were collected at 10 geographical locations, spanning spatial distances of up to 640 km, transecting the oceanic polar front in the Barents Sea. The northern sampling sites were generally colder, less saline, shallower, and showed higher concentrations of freshly sedimented phytopigments compared to the southern study locations. Sampling sites depicted low variation in relative abundances of taxa at class level, with persistent numerical dominance by lineages of Gamma- and Deltaproteobacteria (57-66% of bacterial sequence reads). The Archaea, which constituted 0.7-1.8% of 16S rRNA gene copy numbers in the sediment, were overwhelmingly (85.8%) affiliated with the Thaumarchaeota. Beta-diversity analyses showed the environmental variations throughout the sampling range to have a stronger impact on the structuring of both the bacterial and archaeal communities than spatial effects. While bacterial communities were significantly influenced by the combined effect of several weakly selective environmental differences, including temperature, archaeal communities appeared to be more uniquely structured by the level of freshly sedimented phytopigments.
Keywords: 16S rRNA gene; Barents Sea; archaea; bacteria; beta-diversity; sediment
References
- Appl Environ Microbiol. 2001 Jan;67(1):387-95 - PubMed
- Appl Environ Microbiol. 2003 May;69(5):2463-83 - PubMed
- Appl Environ Microbiol. 2003 Dec;69(12):7224-35 - PubMed
- Appl Environ Microbiol. 2004 Feb;70(2):781-9 - PubMed
- Nature. 2004 Dec 9;432(7018):750-3 - PubMed
- Appl Environ Microbiol. 2005 Jan;71(1):467-79 - PubMed
- Nature. 2005 Sep 22;437(7058):543-6 - PubMed
- Nat Rev Microbiol. 2006 Feb;4(2):102-12 - PubMed
- Environ Microbiol. 2006 Apr;8(4):732-40 - PubMed
- Trends Microbiol. 2006 Jun;14(6):257-63 - PubMed
- Appl Environ Microbiol. 2006 Jul;72(7):4931-41 - PubMed
- Appl Environ Microbiol. 2006 Jul;72(7):5069-72 - PubMed
- BMC Bioinformatics. 2006 Aug 07;7:371 - PubMed
- FEMS Microbiol Ecol. 2007 Sep;61(3):496-508 - PubMed
- Genome Biol. 2007;8(7):R143 - PubMed
- Nat Rev Microbiol. 2007 Oct;5(10):770-81 - PubMed
- FEMS Microbiol Ecol. 2007 Dec;62(3):242-57 - PubMed
- Nat Rev Microbiol. 2008 Mar;6(3):245-52 - PubMed
- Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7774-8 - PubMed
- Nucleic Acids Res. 2009 Jan;37(Database issue):D489-93 - PubMed
- Extremophiles. 2009 Mar;13(2):233-46 - PubMed
- ISME J. 2009 Jul;3(7):860-9 - PubMed
- ISME J. 2009 Jul;3(7):780-91 - PubMed
- Nat Rev Microbiol. 2009 Jun;7(6):451-9 - PubMed
- ISME J. 2009 Nov;3(11):1269-85 - PubMed
- Appl Environ Microbiol. 2009 Aug;75(16):5227-36 - PubMed
- Environ Microbiol. 2009 Dec;11(12):3132-9 - PubMed
- Environ Microbiol. 2010 Jan;12(1):118-23 - PubMed
- ISME J. 2010 Feb;4(2):159-70 - PubMed
- Bioinformatics. 2010 Jan 15;26(2):266-7 - PubMed
- Environ Microbiol. 2010 Jul;12(7):1889-98 - PubMed
- Nat Methods. 2010 May;7(5):335-6 - PubMed
- FEMS Microbiol Ecol. 2010 Jun;72(3):370-85 - PubMed
- Bioinformatics. 2010 Oct 1;26(19):2460-1 - PubMed
- FEMS Microbiol Ecol. 2010 Nov;74(2):410-29 - PubMed
- Nat Methods. 2010 Sep;7(9):668-9 - PubMed
- Adv Microb Physiol. 2010;57:1-41 - PubMed
- ISME J. 2011 May;5(5):879-95 - PubMed
- Mol Ecol. 2011 Jan;20(2):258-74 - PubMed
- Genome Res. 2011 Mar;21(3):494-504 - PubMed
- Appl Environ Microbiol. 2011 Mar;77(6):2008-18 - PubMed
- J Microbiol Methods. 2011 Jul;86(1):42-51 - PubMed
- Proc Natl Acad Sci U S A. 2011 May 10;108(19):7850-4 - PubMed
- Curr Opin Microbiol. 2011 Jun;14(3):300-6 - PubMed
- Environ Microbiol. 2011 Dec;13(12):3219-34 - PubMed
- PLoS One. 2011;6(9):e24570 - PubMed
- ISME J. 2012 Mar;6(3):513-23 - PubMed
- ISME J. 2012 Apr;6(4):724-32 - PubMed
- Nat Rev Microbiol. 2012 May 14;10(7):497-506 - PubMed
- Front Microbiol. 2012 May 31;3:168 - PubMed
- ISME J. 2012 Nov;6(11):2014-23 - PubMed
- ISME J. 2013 Apr;7(4):685-96 - PubMed
- ISME J. 2013 Jul;7(7):1310-21 - PubMed
- Microbiologyopen. 2013 Aug;2(4):541-52 - PubMed
- Environ Microbiol Rep. 2012 Feb;4(1):1-9 - PubMed
- Environ Microbiol Rep. 2011 Dec;3(6):689-97 - PubMed
- Nature. 2013 Jul 25;499(7459):431-7 - PubMed
- PLoS One. 2013 Sep 02;8(9):e72779 - PubMed
- Mol Ecol. 2014 Feb;23(4):954-64 - PubMed
- ISME J. 2014 Nov;8(11):2167-79 - PubMed
- Extremophiles. 2014 Sep;18(5):865-75 - PubMed
- ISME J. 2015 Mar 17;9(4):990-1002 - PubMed
- Oecologia. 2001 Oct;129(2):271-280 - PubMed
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