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Gillette R, Miller-Crews I, Skinner MK, et al. Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat. Front Genet. 2015;6:56doi: 10.3389/fgene.2015.00056.
Gillette, R., Miller-Crews, I., Skinner, M. K., & Crews, D. (2015). Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat. Frontiers in genetics, 656. https://doi.org/10.3389/fgene.2015.00056
Gillette, Ross, et al. "Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat." Frontiers in genetics vol. 6 (2015): 56. doi: https://doi.org/10.3389/fgene.2015.00056
Gillette R, Miller-Crews I, Skinner MK, Crews D. Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat. Front Genet. 2015 Mar 02;6:56. doi: 10.3389/fgene.2015.00056. eCollection 2015. PMID: 25784924; PMCID: PMC4345841.
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Front Genet. 2015 Mar 02;6:56. doi: 10.3389/fgene.2015.00056. eCollection 2015.

Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat.

Frontiers in genetics

Ross Gillette, Isaac Miller-Crews, Michael K Skinner, David Crews

Affiliations

  1. Institute for Cellular and Molecular Biology, The University of Texas at Austin Austin, TX, USA.
  2. Center for Reproductive Biology, School of Biological Sciences, Washington State University Pullman, WA, USA.
  3. Institute for Cellular and Molecular Biology, The University of Texas at Austin Austin, TX, USA ; Department of Integrative Biology, The University of Texas at Austin Austin, TX, USA.

PMID: 25784924 PMCID: PMC4345841 DOI: 10.3389/fgene.2015.00056

Abstract

Exposure to the endocrine disrupting chemical vinclozolin during gestation of an F0 generation and/or chronic restraint stress during adolescence of the F3 descendants affects behavior, physiology, and gene expression in the brain. Genes related to the networks of growth factors, signaling peptides, and receptors, steroid hormone receptors and enzymes, and epigenetic related factors were measured using quantitative polymerase chain reaction via Taqman low density arrays targeting 48 genes in the central amygdaloid nucleus, medial amygdaloid nucleus, medial preoptic area (mPOA), lateral hypothalamus (LH), and the ventromedial nucleus of the hypothalamus. We found that growth factors are particularly vulnerable to ancestral exposure in the central and medial amygdala; restraint stress during adolescence affected neural growth factors in the medial amygdala. Signaling peptides were affected by both ancestral exposure and stress during adolescence primarily in hypothalamic nuclei. Steroid hormone receptors and enzymes were strongly affected by restraint stress in the mPOA. Epigenetic related genes were affected by stress in the ventromedial nucleus and by both ancestral exposure and stress during adolescence independently in the central amygdala. It is noteworthy that the LH showed no effects of either manipulation. Gene expression is discussed in the context of behavioral and physiological measures previously published.

Keywords: amygdala; endocrine disruption; hypothalamus; transgenerational; vinclozolin

References

  1. Hippocampus. 2004;14(5):636-48 - PubMed
  2. Int J Epidemiol. 2012 Feb;41(1):223-9 - PubMed
  3. PLoS One. 2008;3(11):e3745 - PubMed
  4. J Neurosci. 2010 Jun 16;30(24):8308-19 - PubMed
  5. Proc Natl Acad Sci U S A. 2012 Jun 5;109 (23 ):9143-8 - PubMed
  6. J Neurosci. 2012 Nov 28;32(48):17059-66 - PubMed
  7. J Neurosci. 1997 Jul 1;17(13):5245-53 - PubMed
  8. Neuron. 2007 Mar 15;53(6):857-69 - PubMed
  9. Am J Physiol Regul Integr Comp Physiol. 2001 Aug;281(2):R444-51 - PubMed
  10. PLoS One. 2013;8(1):e55387 - PubMed
  11. J Neurosci. 2011 Feb 2;31(5):1873-84 - PubMed
  12. Brain Cogn. 2010 Feb;72(1):73-85 - PubMed
  13. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):726-30 - PubMed
  14. Physiol Behav. 2012 Feb 28;105(4):966-74 - PubMed
  15. Cell. 1999 Oct 29;99(3):247-57 - PubMed
  16. Physiol Behav. 1975 Sep;15(3):323-31 - PubMed
  17. Glia. 2003 May;42(3):263-74 - PubMed
  18. Annu Rev Neurosci. 1992;15:353-75 - PubMed
  19. Brain Res. 1982 May 20;240(1):27-41 - PubMed
  20. Neuroscience. 2012 Dec 13;226:459-74 - PubMed
  21. Evolution. 2012 Jan;66(1):1-17 - PubMed
  22. Nature. 1993 Nov 25;366(6453):362-5 - PubMed
  23. Prog Neurobiol. 1992;38(5):423-53 - PubMed
  24. Toxicol Appl Pharmacol. 1994 Jun;126(2):276-85 - PubMed
  25. Neuroscience. 2003;116(1):1-6 - PubMed
  26. Neuroendocrinology. 1991 Jun;53(6):543-8 - PubMed
  27. Birth Defects Res A Clin Mol Teratol. 2005 Jul;73(7):478-80 - PubMed
  28. Braz J Med Biol Res. 2000 Jan;33(1):79-83 - PubMed
  29. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6114-8 - PubMed
  30. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14 ):5942-6 - PubMed
  31. Behav Neurosci. 2004 Apr;118(2):324-32 - PubMed
  32. Endocr Rev. 2009 Jun;30(4):293-342 - PubMed
  33. Mol Cell Biol. 1998 Nov;18(11):6538-47 - PubMed
  34. F1000 Biol Rep. 2012;4:18 - PubMed
  35. J Comp Neurol. 2003 Mar 10;457(3):213-35 - PubMed
  36. Mol Cell Endocrinol. 2014 Dec;398(1-2):42-52 - PubMed
  37. J Neurosci. 2002 Aug 1;22(15):6810-8 - PubMed
  38. Endocrinology. 2006 Dec;147(12 ):5515-23 - PubMed
  39. Endocrinology. 2013 Jun;154(6):2129-43 - PubMed
  40. Endocrinology. 2014 Oct;155(10 ):3853-66 - PubMed
  41. J Biol Chem. 1995 Aug 25;270(34):19998-20003 - PubMed
  42. Annu Rev Neurosci. 1999;22:295-318 - PubMed
  43. Physiol Rev. 2003 Jul;83(3):803-34 - PubMed
  44. Physiol Behav. 2004 Jun;81(4):671-80 - PubMed
  45. J Androl. 2006 Nov-Dec;27(6):868-79 - PubMed
  46. Horm Behav. 2012 Jun;62(1):50-7 - PubMed
  47. Horm Behav. 2011 Mar;59(3):393-8 - PubMed
  48. Cell. 1998 Feb 20;92(4):437-40 - PubMed
  49. Mol Cell Biol. 2013 Oct;33(20):4106-15 - PubMed
  50. Diagn Pathol. 2014 Jul 10;9:141 - PubMed
  51. Neuroscience. 2005;133(4):903-10 - PubMed
  52. Neuroscience. 2000;96(4):825-36 - PubMed
  53. Dev Psychobiol. 2010 Apr;52(3):244-53 - PubMed
  54. J Neurosci. 2006 Jul 5;26(27):7189-200 - PubMed

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