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Huang B, Wang Z, Park JH, et al. Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Nutr Res Pract. 2015;9(1):22-9doi: 10.4162/nrp.2015.9.1.22.
Huang, B., Wang, Z., Park, J. H., Ryu, O. H., Choi, M. K., Lee, J. Y., Kang, Y. H., & Lim, S. S. (2015). Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Nutrition research and practice, 9(1), 22-9. https://doi.org/10.4162/nrp.2015.9.1.22
Huang, Bo, et al. "Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice." Nutrition research and practice vol. 9,1 (2015): 22-9. doi: https://doi.org/10.4162/nrp.2015.9.1.22
Huang B, Wang Z, Park JH, Ryu OH, Choi MK, Lee JY, Kang YH, Lim SS. Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Nutr Res Pract. 2015 Feb;9(1):22-9. doi: 10.4162/nrp.2015.9.1.22. Epub 2015 Jan 28. PMID: 25671064; PMCID: PMC4317475.
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Nutr Res Pract. 2015 Feb;9(1):22-9. doi: 10.4162/nrp.2015.9.1.22. Epub 2015 Jan 28.

Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice.

Nutrition research and practice

Bo Huang, Zhiqiang Wang, Jong Hyuk Park, Ok Hyun Ryu, Moon Ki Choi, Jae-Yong Lee, Young-Hee Kang, Soon Sung Lim

Affiliations

  1. College of Food Science and Engineering, Liaoning Medical University, Jinzhou 121000, China.
  2. Department of Food Science and Nutrition and Center for Aging and HealthCare, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon 200-702, Korea.
  3. Institute of Natural Medicine, Hallym University Medical School, Gangwon 200-702, Korea.
  4. Division of Endocrinology and Metabolism, Department of Internal Medicine, Hallym University, Gangwon 200-702, Korea.
  5. Institute of Natural Medicine, Hallym University Medical School, Gangwon 200-702, Korea. ; Department of Biochemistry, School of Medicine, Hallym University, Gangwon 200-702, Korea.
  6. Institute of Natural Medicine, Hallym University Medical School, Gangwon 200-702, Korea. ; Department of Food Science and Nutrition and Center for Aging and HealthCare, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon 200-702, Korea.

PMID: 25671064 PMCID: PMC4317475 DOI: 10.4162/nrp.2015.9.1.22

Abstract

BACKGROUND/OBJECTIVES: Recently, anthocyanins have been reported to have various biological activities. Furthermore, anthocyanin-rich purple corn extract (PCE) ameliorated insulin resistance and reduced diabetes-associated mesanginal fibrosis and inflammation, suggesting that it may have benefits for the prevention of diabetes and diabetes complications. In this study, we determined the anthocyanins and non-anthocyanin component of PCE by HPLC-ESI-MS and investigated its anti-diabetic activity and mechanisms using C57BL/KsJ db/db mice.

MATERIALS/METHODS: The db/db mice were divided into four groups: diabetic control group (DC), 10 or 50 mg/kg PCE (PCE 10 or PCE 50), or 10 mg/kg pinitol (pinitol 10) and treated with drugs once per day for 8 weeks. During the experiment, body weight and blood glucose levels were measured every week. At the end of treatment, we measured several diabetic parameters.

RESULTS: Compared to the DC group, Fasting blood glucose levels were 68% lower in PCE 50 group and 51% lower in the pinitol 10 group. Furthermore, the PCE 50 group showed 2- fold increased C-peptide and adiponectin levels and 20% decreased HbA1c levels, than in the DC group. In pancreatic islets morphology, the PCE- or pinitol-treated mice showed significant prevention of pancreatic β-cell damage and higher insulin content. Microarray analyses results indicating that gene and protein expressions associated with glycolysis and fatty acid metabolism in liver and fat tissues. In addition, purple corn extract increased the phosphorylation of AMP-activated protein kinase (AMPK) and decreased phosphoenolpyruvate carboxykinase (PEPCK), glucose 6-phosphatase (G6pase) genes in liver, and also increased glucose transporter 4 (GLUT4) expressions in skeletal muscle.

CONCLUSIONS: Our results suggested that PCE exerted anti-diabetic effects through protection of pancreatic β-cells, increase of insulin secretion and AMPK activation in the liver of C57BL/KsJ db/db mice.

Keywords: AMPK; Purple corn; diabetes; insulin; microarray assay

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