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Seron-Ferre M, Reynolds H, Mendez NA, et al. Impact of Maternal Melatonin Suppression on Amount and Functionality of Brown Adipose Tissue (BAT) in the Newborn Sheep. Front Endocrinol (Lausanne). 2015;5:232doi: 10.3389/fendo.2014.00232.
Seron-Ferre, M., Reynolds, H., Mendez, N. A., Mondaca, M., Valenzuela, F., Ebensperger, R., Valenzuela, G. J., Herrera, E. A., Llanos, A. J., & Torres-Farfan, C. (2014). Impact of Maternal Melatonin Suppression on Amount and Functionality of Brown Adipose Tissue (BAT) in the Newborn Sheep. Frontiers in endocrinology, 5232. https://doi.org/10.3389/fendo.2014.00232
Seron-Ferre, Maria, et al. "Impact of Maternal Melatonin Suppression on Amount and Functionality of Brown Adipose Tissue (BAT) in the Newborn Sheep." Frontiers in endocrinology vol. 5 (2014): 232. doi: https://doi.org/10.3389/fendo.2014.00232
Seron-Ferre M, Reynolds H, Mendez NA, Mondaca M, Valenzuela F, Ebensperger R, Valenzuela GJ, Herrera EA, Llanos AJ, Torres-Farfan C. Impact of Maternal Melatonin Suppression on Amount and Functionality of Brown Adipose Tissue (BAT) in the Newborn Sheep. Front Endocrinol (Lausanne). 2015 Jan 06;5:232. doi: 10.3389/fendo.2014.00232. eCollection 2014. PMID: 25610428; PMCID: PMC4285176.
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Front Endocrinol (Lausanne). 2015 Jan 06;5:232. doi: 10.3389/fendo.2014.00232. eCollection 2014.

Impact of Maternal Melatonin Suppression on Amount and Functionality of Brown Adipose Tissue (BAT) in the Newborn Sheep.

Frontiers in endocrinology

Maria Seron-Ferre, Henry Reynolds, Natalia Andrea Mendez, Mauricio Mondaca, Francisco Valenzuela, Renato Ebensperger, Guillermo J Valenzuela, Emilio A Herrera, Anibal J Llanos, Claudia Torres-Farfan

Affiliations

  1. Facultad de Medicina, Laboratorio de Cronobiología, Instituto de Ciencias Biomédicas (ICBM), Universidad de Chile , Santiago , Chile ; Programa de Fisiopatología, Facultad de Medicina, Instituto de Ciencias Biomédicas (ICBM), Universidad de Chile , Santiago , Chile.
  2. Facultad de Medicina, Laboratorio de Cronobiología del Desarrollo, Universidad Austral de Chile , Valdivia , Chile.
  3. Department of Women's Health, Arrowhead Regional Medical Center , San Bernardino, CA , USA.
  4. Programa de Fisiopatología, Facultad de Medicina, Instituto de Ciencias Biomédicas (ICBM), Universidad de Chile , Santiago , Chile.

PMID: 25610428 PMCID: PMC4285176 DOI: 10.3389/fendo.2014.00232

Abstract

In human and sheep newborns, brown adipose tissue (BAT) accrued during fetal development is used for newborn thermogenesis. Here, we explored the role of maternal melatonin during gestation on the amount and functionality of BAT in the neonate. We studied BAT from six lambs gestated by ewes exposed to constant light from 63% gestation until delivery to suppress melatonin (LL), six lambs gestated by ewes exposed to LL but receiving daily oral melatonin (12 mg at 1700 h, LL + Mel) and another six control lambs gestated by ewes maintained in 12 h light:12 h dark (LD). Lambs were instrumented at 2 days of age. At 4-6 days of age, they were exposed to 24°C (thermal neutrality conditions) for 1 h, 4°C for 1 h, and 24°C for 1 h. Afterward, lambs were euthanized and BAT was dissected for mRNA measurement, histology, and ex vivo experiments. LL newborns had lower central BAT and skin temperature under thermal neutrality and at 4°C, and higher plasma norepinephrine concentration than LD newborns. In response to 4°C, they had a pronounced decrease in skin temperature and did not increase plasma glycerol. BAT weight in LL newborns was about half of that of LD newborns. Ex vivo, BAT from LL newborns showed increased basal lipolysis and did not respond to NE. In addition, expression of adipogenic/thermogenic genes (UCP1, ADBR3, PPARγ, PPARα, PGC1α, C/EBPβ, and perilipin) and of the clock genes Bmal1, Clock, and Per2 was increased. Remarkably, the effects observed in LL newborns were absent in LL + Mel newborns. Thus, our results support that maternal melatonin during gestation is important in determining amount and normal functionality of BAT in the neonate.

Keywords: SCN; circadian; constant light; development; newborn; thermoregulation

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