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Pejchar P, Potocký M, Krčková Z, et al. Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana. Front Plant Sci. 2015;6:66doi: 10.3389/fpls.2015.00066.
Pejchar, P., Potocký, M., Krčková, Z., Brouzdová, J., Daněk, M., & Martinec, J. (2015). Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana. Frontiers in plant science, 666. https://doi.org/10.3389/fpls.2015.00066
Pejchar, Přemysl, et al. "Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana." Frontiers in plant science vol. 6 (2015): 66. doi: https://doi.org/10.3389/fpls.2015.00066
Pejchar P, Potocký M, Krčková Z, Brouzdová J, Daněk M, Martinec J. Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana. Front Plant Sci. 2015 Feb 16;6:66. doi: 10.3389/fpls.2015.00066. eCollection 2015. PMID: 25763003; PMCID: PMC4329606.
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Front Plant Sci. 2015 Feb 16;6:66. doi: 10.3389/fpls.2015.00066. eCollection 2015.

Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana.

Frontiers in plant science

Přemysl Pejchar, Martin Potocký, Zuzana Krčková, Jitka Brouzdová, Michal Daněk, Jan Martinec

Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague Czech Republic.

PMID: 25763003 PMCID: PMC4329606 DOI: 10.3389/fpls.2015.00066

Abstract

Aluminum ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. The first indication of the Al toxicity in plants is the cessation of root growth, but the mechanism of root growth inhibition is largely unknown. Here we examined the impact of Al on the expression, activity, and function of the non-specific phospholipase C4 (NPC4), a plasma membrane-bound isoform of NPC, a member of the plant phospholipase family, in Arabidopsis thaliana. We observed a lower expression of NPC4 using β-glucuronidase assay and a decreased formation of labeled diacylglycerol, product of NPC activity, using fluorescently labeled phosphatidylcholine as a phospholipase substrate in Arabidopsis WT seedlings treated with AlCl3 for 2 h. The effect on in situ NPC activity persisted for longer Al treatment periods (8, 14 h). Interestingly, in seedlings overexpressing NPC4, the Al-mediated NPC-inhibiting effect was alleviated at 14 h. However, in vitro activity and localization of NPC4 were not affected by Al, thus excluding direct inhibition by Al ions or possible translocation of NPC4 as the mechanisms involved in NPC-inhibiting effect. Furthermore, the growth of tobacco pollen tubes rapidly arrested by Al was partially rescued by the overexpression of AtNPC4 while Arabidopsis npc4 knockout lines were found to be more sensitive to Al stress during long-term exposure of Al at low phosphate conditions. Our observations suggest that NPC4 plays a role in both early and long-term responses to Al stress.

Keywords: Arabidopsis; aluminum toxicity; diacylglycerol; non-specific phospholipase C; plasma membrane; pollen tube; signaling; tobacco

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