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Malakar P, Singh VK, Karmakar R, et al. Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli. Springerplus. 2014;3:748doi: 10.1186/2193-1801-3-748.
Malakar, P., Singh, V. K., Karmakar, R., & Venkatesh, K. V. (2014). Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli. SpringerPlus, 3748. https://doi.org/10.1186/2193-1801-3-748
Malakar, Pushkar, et al. "Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli." SpringerPlus vol. 3 (2014): 748. doi: https://doi.org/10.1186/2193-1801-3-748
Malakar P, Singh VK, Karmakar R, Venkatesh KV. Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli. Springerplus. 2014 Dec 17;3:748. doi: 10.1186/2193-1801-3-748. eCollection 2014. PMID: 25674477; PMCID: PMC4320194.
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Springerplus. 2014 Dec 17;3:748. doi: 10.1186/2193-1801-3-748. eCollection 2014.

Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli.

SpringerPlus

Pushkar Malakar, Vivek K Singh, Richa Karmakar, Kareenhalli V Venkatesh

Affiliations

  1. Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
  2. Department of Chemical Engineering, IIT Bombay, Mumbai, 400076 India.
  3. Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India ; Department of Chemical Engineering, IIT Bombay, Mumbai, 400076 India.

PMID: 25674477 PMCID: PMC4320194 DOI: 10.1186/2193-1801-3-748

Abstract

Osmotic Shock is known to negatively affect growth rate along with an extended lag phase. The reduction in growth rate can be characterized as burden due to the osmotic stress. Studies have shown that production of unnecessary protein also burdens cellular growth. This has been demonstrated by growing Escherichia coli on glycerol in the presence of Isopropyl-β-D-1-thiogalactopyranoside (IPTG) to induce β-galactosidase synthesis which does not offer any benefit towards growth. The trade off between osmotic stress and burden on growth due to unnecessary gene expression has not been enumerated. The influence of osmotic stress on β-galactosidase synthesis and activity is not clearly understood. Here, we study the effect of salt concentration on β-galactosidase activity and burden on growth due to unnecessary gene expression in E.coli. We characterize the burden on growth in presence of varying concentrations of salt in the presence of IPTG using three strains, namely wild type, ∆lacI and ∆lacIlacZ mutant strains. We demonstrate that the salt concentrations, sensitively inhibits enzyme synthesis thereby influencing the burden on growth. In a wild type strain, addition of lactose into the medium demonstrated growth benefit at low salt concentration but not at higher concentrations. The extent of burden due to osmotic shock was higher in a lactose M9 medium than in a glycerol M9 medium. A linear relationship was observed between enzyme activity and burden on growth in various media types studied.

Keywords: Escherichia coli; Growth rate; IPTG; Osmotic stress; Unnecessary Gene Expression

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