Display options
Cite
Oner M, Dulgeroglu TC, Karaman I, et al. The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model. Curr Ther Res Clin Exp. 2015;77:35-9doi: 10.1016/j.curtheres.2015.01.002.
Oner, M., Dulgeroglu, T. C., Karaman, I., Guney, A., Kafadar, I. H., & Erdem, S. (2015). The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model. Current therapeutic research, clinical and experimental, 7735-9. https://doi.org/10.1016/j.curtheres.2015.01.002
Oner, Mithat, et al. "The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model." Current therapeutic research, clinical and experimental vol. 77 (2015): 35-9. doi: https://doi.org/10.1016/j.curtheres.2015.01.002
Oner M, Dulgeroglu TC, Karaman I, Guney A, Kafadar IH, Erdem S. The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model. Curr Ther Res Clin Exp. 2015 Feb 03;77:35-9. doi: 10.1016/j.curtheres.2015.01.002. eCollection 2015 Dec. PMID: 25737745; PMCID: PMC4339529.
Copy Download .nbib Format: NLM
Share it on

Curr Ther Res Clin Exp. 2015 Feb 03;77:35-9. doi: 10.1016/j.curtheres.2015.01.002. eCollection 2015 Dec.

The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model.

Current therapeutic research, clinical and experimental

Mithat Oner, Turan Cihan Dulgeroglu, Ibrahim Karaman, Ahmet Guney, Ibrahim Halil Kafadar, Sevki Erdem

Affiliations

  1. Department of Orthopaedics and Traumatology, Erciyes University Medical Faculty, Kayseri, Turkey.
  2. Department of Orthopaedics and Traumatology, Dumlup?nar University Medical Faculty, Kütahya, Turkey.
  3. Department of Orthopaedics and Traumatology, Medeniyet University Medical Faculty, Istanbul, Turkey.

PMID: 25737745 PMCID: PMC4339529 DOI: 10.1016/j.curtheres.2015.01.002

Abstract

OBJECTIVE: The high risk of nonunion represents a challenge in vertebral surgery, thus stimulating new strategies to improve fusion rates. We investigated the effect of 2 different bone grafts and amniotic fluid application on radiologically and histologically evaluated vertebral fusion in an experimental rat model.

MATERIALS AND METHODS: Forty-eight 24-week-old Sprague Dawley rats were included and assigned into 1 of 4 groups: allograft group, allograft plus human amniotic fluid group, demineralized bone matrix (DBM) group, or DBM plus human amniotic fluid group. After decortication and L4-L6 spinal fusion, study treatments were applied. Fusion in each rat was examined radiologically and histologically 8 weeks after the intervention.

RESULTS: The group that received only allograft had better radiologic scores (median = 3.5; range = 3-4) when compared with the group that received only DBM (median = 2; range = 1-4) (P = 0.002); however, histologic scores did not differ. When amniotic fluid was added to the grafting, allograft-based treatments performed better than DBM-based treatments both on radiologic (median = 4; range = 3-4 vs median = 3; range = 3-4; P = 0.003) and histologic (median = 7; range = 6-7 vs median = 5; range = 3-6; P < 0.001) evaluation. Addition of amniotic fluid did not result in better outcomes in the rats that received DBM-based treatments but based on histologic evaluation, rats that received allograft-based treatments benefited from this application.

CONCLUSIONS: Amniotic fluid seems to have an enhancing effect on posterior spinal fusion, particularly when combined with allograft.

Keywords: Allograft; amniotic fluid; demineralized bone matrix; experimental study; vertebral fusion

References

  1. J Clin Endocrinol Metab. 1984 Oct;59(4):752-5 - PubMed
  2. Spine (Phila Pa 1976). 1993 Jul;18(9):1123-4 - PubMed
  3. Spine (Phila Pa 1976). 1998 Jan 15;23 (2):159-67 - PubMed
  4. J Bone Joint Surg Am. 2004 Oct;86-A(10):2243-50 - PubMed
  5. Clin Orthop Relat Res. 1967 Jul-Aug;53:243-83 - PubMed
  6. Br J Plast Surg. 2002 Apr;55(3):246-50 - PubMed
  7. Clin Orthop Relat Res. 2004 Aug;(425):135-42 - PubMed
  8. Clin Orthop Relat Res. 1976 Sep;(119):231-6 - PubMed
  9. J Surg Res. 2005 Dec;129(2):283-7 - PubMed
  10. J Bone Joint Surg Am. 1994 Apr;76(4):540-8 - PubMed
  11. J Bone Joint Surg Br. 2001 Mar;83(2):157-64 - PubMed
  12. Spine (Phila Pa 1976). 1995 Jun 15;20(12 ):1410-8 - PubMed
  13. World J Orthop. 2014 Jan 18;5(1):30-7 - PubMed
  14. Knee. 2005 Apr;12(2):113-9 - PubMed
  15. Spine (Phila Pa 1976). 1995 Dec 15;20(24 Suppl):138S-142S - PubMed
  16. J Spinal Disord. 1992 Dec;5(4):433-42 - PubMed
  17. Eklem Hastalik Cerrahisi. 2011 Dec;22(3):166-71 - PubMed
  18. Surgeon. 2013 Feb;11(1):39-48 - PubMed
  19. Br J Plast Surg. 2004 Jul;57(5):423-8 - PubMed

Publication Types