Display options
Cite
Kasempimolporn S, Thaveekarn W, Kerdpanich P, et al. Performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children. J Clin Diagn Res. 2015;9(1):DC11-4doi: 10.7860/JCDR/2015/10989.5403.
Kasempimolporn, S., Thaveekarn, W., Kerdpanich, P., Skulpichetrat, U., Saekhow, O., Boonchang, S., Bharnthong, T., & Sitprija, V. (2015). Performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children. Journal of clinical and diagnostic research : JCDR, 9(1), DC11-4. https://doi.org/10.7860/JCDR/2015/10989.5403
Kasempimolporn, Songsri, et al. "Performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children." Journal of clinical and diagnostic research : JCDR vol. 9,1 (2015): DC11-4. doi: https://doi.org/10.7860/JCDR/2015/10989.5403
Kasempimolporn S, Thaveekarn W, Kerdpanich P, Skulpichetrat U, Saekhow O, Boonchang S, Bharnthong T, Sitprija V. Performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children. J Clin Diagn Res. 2015 Jan;9(1):DC11-4. doi: 10.7860/JCDR/2015/10989.5403. Epub 2015 Jan 01. PMID: 25737986; PMCID: PMC4347077.
Copy Download .nbib Format: NLM
Share it on

J Clin Diagn Res. 2015 Jan;9(1):DC11-4. doi: 10.7860/JCDR/2015/10989.5403. Epub 2015 Jan 01.

Performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children.

Journal of clinical and diagnostic research : JCDR

Songsri Kasempimolporn, Wichit Thaveekarn, Phirangkul Kerdpanich, Urailak Skulpichetrat, Orawan Saekhow, Supatsorn Boonchang, Thipchuta Bharnthong, Visith Sitprija

Affiliations

  1. Advisory Scientist, Department of Research and Development, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .
  2. Scientist, Department of Research and Development, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .
  3. Associate Professor, Infectious Disease Unit, Department of Pediatrics, Phramongkutklao Hospital , Bangkok, Thailand .
  4. Pediatric Fellow, Infectious Disease Unit, Department of Pediatrics, Phramongkutklao Hospital , Bangkok, Thailand .
  5. Senior Scientist, Department of Research and Development, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .
  6. Laboratory Officer, Department of Research and Development, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .
  7. Former Head, Department of BCG Vaccine Production, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .
  8. Director Professor, Queen Saovabha Memorial Institute , Thai Red Cross Society, Bangkok, Thailand .

PMID: 25737986 PMCID: PMC4347077 DOI: 10.7860/JCDR/2015/10989.5403

Abstract

BACKGROUND: The serodiagnostic tests for tuberculosis (TB) present a high variability in terms of sensitivity and specificity. Data on patients with latent TB infection (LTBI) and children in high prevalence settings are still limited. The present study aimed to evaluate an in-house strip test for detection of anti-M. tuberculosis antibodies in TB patients, mostly children aged under 15 y, grouped into four diagnostic categories: active TB, LTBI, healthy TB contacts, and other non-TB diseases.

MATERIALS AND METHODS: The diagnostic performance of strip test was compared with the tuberculin skin test (TST) and interferon-gamma release assay (IGRA). Sensitivity and specificity were assessed for all three diagnostic tests. The detection accuracy among the tests was calculated by using a receiver operating characteristic analysis.

RESULTS: TST and IGRA could diagnose the active TB cases correctly (100%). The sensitivity of strip test for active TB was 58.3% and 37.5% for LTBI, while the sensitivities of TST and IGRA for LTBI were 90.3% and 37.5%, respectively. The overall specificities of strip test and IGRA were 91.5% and 95.7%, respectively, which were superior to that of TST (68.1%).

CONCLUSION: The strip test did not appear to be useful for diagnosis of active TB in comparison with the current diagnostic standard. The assay may be particularly significant in situations where TB is clinically difficult to diagnose like LTBI and could be a meaningful tool in terms of high specificity and simplicity for ruling in pediatric TB in countries with high TB infection rate. Further studies are needed to determine whether strip test can be improved in its sensitivity and should be implemented into routine clinical practice.

Keywords: Immunochromatographic test; Interferon-gamma release assay; Latent tuberculosis; Serodiagnosis; Tuberculin skin test

References

  1. J Clin Microbiol. 2011 Aug;49(8):3061-4 - PubMed
  2. Clin Exp Immunol. 2008 Apr;152(1):81-7 - PubMed
  3. Clin Diagn Lab Immunol. 2000 Mar;7(2):155-60 - PubMed
  4. Clin Vaccine Immunol. 2006 Feb;13(2):179-86 - PubMed
  5. PLoS One. 2014 Jul 31;9(7):e103811 - PubMed
  6. Infect Immun. 2005 Oct;73(10):6846-51 - PubMed
  7. Clin Infect Dis. 2005 Jan 15;40(2):211-7 - PubMed
  8. Mol Cell Proteomics. 2006 Nov;5(11):2102-13 - PubMed
  9. Eur Respir J. 2009 May;33(5):956-73 - PubMed
  10. PLoS One. 2012;7(10):e47340 - PubMed
  11. Proc Am Thorac Soc. 2006;3(1):103-10 - PubMed
  12. PLoS One. 2010 Jun 25;5(6):e11277 - PubMed
  13. Infect Immun. 1994 May;62(5):2058-64 - PubMed
  14. Infect Immun. 2007 Jun;75(6):2914-21 - PubMed
  15. Semin Nucl Med. 1978 Oct;8(4):283-98 - PubMed
  16. Am Rev Respir Dis. 1992 Sep;146(3):752-6 - PubMed
  17. Infect Immun. 2006 Feb;74(2):861-8 - PubMed
  18. Lancet Infect Dis. 2012 Jan;12(1):45-55 - PubMed
  19. J Microbiol Immunol Infect. 2015 Aug;48(4):381-9 - PubMed
  20. Vaccine. 2014 Feb 3;32(6):712-6 - PubMed
  21. Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5730-5 - PubMed
  22. BMC Res Notes. 2012 Aug 07;5:415 - PubMed
  23. Int J Tuberc Lung Dis. 2006 Nov;10(11):1192-204 - PubMed
  24. Ann Intern Med. 2008 Aug 5;149(3):177-84 - PubMed
  25. Infect Immun. 1993 May;61(5):1730-4 - PubMed
  26. Am Rev Respir Dis. 1981 Jul;124(1):80-7 - PubMed
  27. Infect Immun. 2000 Jun;68(6):3314-21 - PubMed
  28. Mol Microbiol. 2004 Jan;51(2):359-70 - PubMed

Publication Types