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Cabibi D, Calvaruso V, Giuffrida L, et al. Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis. Pathobiology. 2015;82(1):48-52doi: 10.1159/000375264.
Cabibi, D., Calvaruso, V., Giuffrida, L., Ingrao, S., Balsamo, L., Giannone, A. G., Petta, S., & Di Marco, V. (2015). Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis. Pathobiology : journal of immunopathology, molecular and cellular biology, 82(1), 48-52. https://doi.org/10.1159/000375264
Cabibi, Daniela, et al. "Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis." Pathobiology : journal of immunopathology, molecular and cellular biology vol. 82,1 (2015): 48-52. doi: https://doi.org/10.1159/000375264
Cabibi D, Calvaruso V, Giuffrida L, Ingrao S, Balsamo L, Giannone AG, Petta S, Di Marco V. Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis. Pathobiology. 2015;82(1):48-52. doi: 10.1159/000375264. Epub 2015 Mar 06. PMID: 25766393.
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Pathobiology. 2015;82(1):48-52. doi: 10.1159/000375264. Epub 2015 Mar 06.

Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis.

Pathobiology : journal of immunopathology, molecular and cellular biology

Daniela Cabibi, Vincenza Calvaruso, Letizia Giuffrida, Sabrina Ingrao, Laura Balsamo, Antonino Giulio Giannone, Salvatore Petta, Vito Di Marco

Affiliations

  1. Human Pathology Section, Department of Science for Promotion of Health and Mother and Child Care, University of Palermo, Palermo, Italy.

PMID: 25766393 DOI: 10.1159/000375264

Abstract

OBJECTIVE: To compare Masson's trichrome (MT), Sirius red (SR) and orcein staining in acute hepatitis (AH) and to correlate them with transient elastography (TE), a noninvasive method to assess hepatic fibrosis.

METHODS: We evaluated liver stiffness by TE in a cohort of 34 consecutive patients and assessed MT-, SR- and orcein-stained biopsies using the METAVIR scoring system and digital image analysis (DIA).

RESULTS: MT and SR both showed severe fibrosis (stage III-IV, DIA = 12.7%). Orcein showed absent or mild fibrosis (stage 0-II, DIA = 4.4%; p < 0.05). In 29/34 cases (85%), stiffness values were >12.5 kPa, in keeping with SR/MT but not with orcein results.

CONCLUSIONS: Even though in AH true elastic fibrosis is typically absent or mild, TE shows elevated stiffness values, in keeping with SR/MT evaluations. If not properly evaluated in the clinical context, these results would lead to an overestimation of fibrosis. Orcein is the only staining able to evidence the absence of true elastic fibrosis, which is a typical feature of AH. This is the first study comparing different staining procedures performed on AH biopsies by DIA versus TE. © 2015 S. Karger AG, Basel.

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