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Lankford L, Selby T, Becker J, et al. Early gestation chorionic villi-derived stromal cells for fetal tissue engineering. World J Stem Cells. 2015;7(1):195-207doi: 10.4252/wjsc.v7.i1.195.
Lankford, L., Selby, T., Becker, J., Ryzhuk, V., Long, C., Farmer, D., & Wang, A. (2015). Early gestation chorionic villi-derived stromal cells for fetal tissue engineering. World journal of stem cells, 7(1), 195-207. https://doi.org/10.4252/wjsc.v7.i1.195
Lankford, Lee, et al. "Early gestation chorionic villi-derived stromal cells for fetal tissue engineering." World journal of stem cells vol. 7,1 (2015): 195-207. doi: https://doi.org/10.4252/wjsc.v7.i1.195
Lankford L, Selby T, Becker J, Ryzhuk V, Long C, Farmer D, Wang A. Early gestation chorionic villi-derived stromal cells for fetal tissue engineering. World J Stem Cells. 2015 Jan 26;7(1):195-207. doi: 10.4252/wjsc.v7.i1.195. PMID: 25621120; PMCID: PMC4300931.
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World J Stem Cells. 2015 Jan 26;7(1):195-207. doi: 10.4252/wjsc.v7.i1.195.

Early gestation chorionic villi-derived stromal cells for fetal tissue engineering.

World journal of stem cells

Lee Lankford, Taryn Selby, James Becker, Volodymyr Ryzhuk, Connor Long, Diana Farmer, Aijun Wang

Affiliations

  1. Lee Lankford, Taryn Selby, James Becker, Volodymyr Ryzhuk, Connor Long, Diana Farmer, Aijun Wang, Department of Surgery, University of California, Davis Health System, Sacramento, CA 95817, United States.

PMID: 25621120 PMCID: PMC4300931 DOI: 10.4252/wjsc.v7.i1.195

Abstract

AIM: To investigate the potential for early gestation placenta-derived mesenchymal stromal cells (PMSCs) for fetal tissue engineering.

METHODS: PMSCs were isolated from early gestation chorionic villus tissue by explant culture. Chorionic villus sampling (CVS)-size tissue samples (mean = 35.93 mg) were used to test the feasibility of obtaining large cell numbers from CVS within a clinically relevant timeframe. We characterized PMSCs isolated from 6 donor placentas by flow cytometry immunophenotyping, multipotency assays, and through immunofluorescent staining. Protein secretion from PMSCs was examined using two cytokine array assays capable of probing for over 70 factors in total. Delivery vehicle compatibility of PMSCs was determined using three common scaffold systems: fibrin glue, collagen hydrogel, and biodegradable nanofibrous scaffolds made from a combination of polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA). Viral transduction of PMSCs was performed using a Luciferase-GFP-containing lentiviral vector and efficiency of transduction was tested by fluorescent microscopy and flow cytometry analysis.

RESULTS: We determined that an average of 2.09 × 10(6) (SD ± 8.59 × 10(5)) PMSCs could be obtained from CVS-size tissue samples within 30 d (mean = 27 d, SD ± 2.28), indicating that therapeutic numbers of cells can be rapidly expanded from very limited masses of tissue. Immunophenotyping by flow cytometry demonstrated that PMSCs were positive for MSC markers CD105, CD90, CD73, CD44, and CD29, and were negative for hematopoietic and endothelial markers CD45, CD34, and CD31. PMSCs displayed trilineage differentiation capability, and were found to express developmental transcription factors Sox10 and Sox17 as well as neural-related structural proteins NFM, Nestin, and S100β. Cytokine arrays revealed a robust and extensive profile of PMSC-secreted cytokines and growth factors, and detected 34 factors with spot density values exceeding 10(3). Detected factors had widely diverse functions that include modulation of angiogenesis and immune response, cell chemotaxis, cell proliferation, blood vessel maturation and homeostasis, modulation of insulin-like growth factor activity, neuroprotection, extracellular matrix degradation and even blood coagulation. Importantly, PMSCs were also determined to be compatible with both biological and synthetic material-based delivery vehicles such as collagen and fibrin hydrogels, and biodegradable nanofiber scaffolds made from a combination of PLA and PLGA. Finally, we demonstrated that PMSCs can be efficiently transduced (> 95%) with a Luciferase-GFP-containing lentiviral vector for future in vivo cell tracking after transplantation.

CONCLUSION: Our findings indicate that PMSCs represent a unique source of cells that can be effectively utilized for in utero cell therapy and tissue engineering.

Keywords: Chorionic villus; Fetal surgery; Mesenchymal stromal cells; Placenta; Tissue engineering

References

  1. Cancer Res. 2006 May 1;66(9):4652-61 - PubMed
  2. Ann N Y Acad Sci. 2002 Dec;979:80-93 - PubMed
  3. Endocr Rev. 1997 Dec;18(6):801-31 - PubMed
  4. Cell Reprogram. 2012 Aug;14(4):334-41 - PubMed
  5. Stem Cells Transl Med. 2012 Feb;1(2):142-9 - PubMed
  6. Circ Res. 2007 Mar 30;100(6):782-94 - PubMed
  7. Soc Sci Med. 1986;23(4):357-66 - PubMed
  8. N Engl J Med. 2011 Mar 17;364(11):993-1004 - PubMed
  9. Blood. 2004 Jul 1;104(1):92-9 - PubMed
  10. Bioessays. 2006 Aug;28(8):788-98 - PubMed
  11. Circ Res. 2008 Nov 21;103(11):1204-19 - PubMed
  12. Circ Res. 2014 Jul 7;115(2):215-26 - PubMed
  13. J Anal Toxicol. 1989 May-Jun;13(3):179-84 - PubMed
  14. PLoS One. 2012;7(4):e34284 - PubMed
  15. Science. 1993 May 14;260(5110):920-6 - PubMed
  16. Stem Cells Int. 2012;2012:658356 - PubMed
  17. J Investig Dermatol Symp Proc. 2000 Dec;5(1):34-9 - PubMed
  18. Thromb Haemost. 2004 Mar;91(3):438-49 - PubMed
  19. Microvasc Res. 2003 Jan;65(1):9-17 - PubMed
  20. J Cell Sci. 1999 Jun;112 ( Pt 12):2049-57 - PubMed
  21. Neuron. 2003 Apr 10;38(1):17-31 - PubMed
  22. Stem Cells. 2006 Apr;24(4):1054-64 - PubMed
  23. Thromb Res. 2008;122 Suppl 1:S42-6 - PubMed
  24. Curr Stem Cell Res Ther. 2012 Jul;7(4):272-81 - PubMed
  25. Prog Growth Factor Res. 1989;1(4):207-35 - PubMed
  26. Cell Prolif. 2012 Feb;45(1):66-75 - PubMed
  27. Am J Kidney Dis. 1986 Dec;8(6):445-9 - PubMed
  28. J Cell Mol Med. 2002 Jan-Mar;6(1):1-12 - PubMed
  29. Am J Obstet Gynecol. 2006 Mar;194(3):664-73 - PubMed
  30. Methods Mol Biol. 2012;879:291-313 - PubMed
  31. Acta Haematol. 2013;129(4):197-206 - PubMed
  32. Biochim Biophys Acta. 1990 Jun 1;1032(1):79-87 - PubMed
  33. Mol Biol (Mosk). 2001 May-Jun;35(3):349-71 - PubMed
  34. Arterioscler Thromb Vasc Biol. 2013 Jul;33(7):1655-62 - PubMed
  35. Cancer Res. 2004 Aug 1;64(15):5283-90 - PubMed
  36. Organogenesis. 2014 Jan 1;10(1):29-37 - PubMed
  37. J Immunol. 2003 Mar 15;170(6):3369-76 - PubMed
  38. BMC Cell Biol. 2010 Jun 23;11:44 - PubMed
  39. Reprod Sci. 2010 Nov;17(11):1006-15 - PubMed
  40. PLoS One. 2014 Jan 29;9(1):e87238 - PubMed
  41. PLoS One. 2012;7(11):e50098 - PubMed
  42. FEBS Lett. 1992 Jul 27;307(1):97-101 - PubMed
  43. Crit Rev Oncol Hematol. 1984;1(3):199-225 - PubMed
  44. Circ Res. 2004 Jul 9;95(1):9-20 - PubMed
  45. PLoS One. 2012;7(9):e43395 - PubMed
  46. Am J Pathol. 2000 Nov;157(5):1703-11 - PubMed
  47. Biochem Biophys Res Commun. 2006 Feb 17;340(3):944-52 - PubMed
  48. J Cell Biochem. 2009 Apr 1;106(5):769-75 - PubMed
  49. Obstet Gynecol. 2005 Sep;106(3):503-8 - PubMed
  50. Circulation. 2006 Jul 4;114(1 Suppl):I125-31 - PubMed
  51. Cytotherapy. 2008;10(7):690-7 - PubMed
  52. Stem Cell Rev. 2013 Feb;9(1):16-31 - PubMed
  53. Cardiovasc Res. 2008 May 1;78(2):203-12 - PubMed
  54. Anticancer Res. 2011 Jun;31(6):2283-90 - PubMed
  55. Bone Marrow Transplant. 2011 Feb;46(2):163-4 - PubMed
  56. BJOG. 2009 Jan;116(2):166-72 - PubMed
  57. Cytotherapy. 2006;8(4):315-7 - PubMed
  58. Nat Rev Mol Cell Biol. 2009 Mar;10(3):165-77 - PubMed
  59. Biochem Biophys Res Commun. 2006 Jun 23;345(1):523-9 - PubMed
  60. Cell. 1997 Jan 24;88(2):277-85 - PubMed
  61. Ann Thorac Surg. 2013 May;95(5):1827-33 - PubMed
  62. J Neurotrauma. 2009 Nov;26(11):1935-41 - PubMed
  63. J Neurochem. 2002 Apr;81(2):365-78 - PubMed
  64. Intern Med J. 2013 Apr;43(4):430-9 - PubMed
  65. Front Biosci (Landmark Ed). 2009 Jan 01;14:3782-94 - PubMed
  66. Pharmacol Rev. 2004 Dec;56(4):549-80 - PubMed
  67. J Cell Biol. 1992 Nov;119(3):629-41 - PubMed
  68. Physiol Rev. 2008 Oct;88(4):1379-406 - PubMed
  69. Science. 1989 Feb 24;243(4894 Pt 1):1074-6 - PubMed
  70. J Cell Physiol. 2009 May;219(2):449-58 - PubMed
  71. Endocr Rev. 2002 Dec;23(6):824-54 - PubMed
  72. J Endocrinol. 2003 Oct;179(1):15-23 - PubMed
  73. Biochim Biophys Acta. 2011 May;1813(5):878-88 - PubMed
  74. Int Immunopharmacol. 2012 Jun;13(2):219-24 - PubMed
  75. J Biosci Bioeng. 2014 Nov;118(5):593-8 - PubMed
  76. Stem Cells Dev. 2011 Mar;20(3):451-63 - PubMed
  77. J Pediatr Surg. 2004 Jun;39(6):834-8; discussion 834-8 - PubMed
  78. Neuron. 1998 Dec;21(6):1291-302 - PubMed
  79. Int J Cancer. 2003 Jun 20;105(3):340-6 - PubMed
  80. Placenta. 2011 Oct;32 Suppl 4:S316-9 - PubMed
  81. Curr Opin Genet Dev. 2003 Aug;13(4):393-400 - PubMed
  82. Future Neurol. 2011 Mar;6(2):165-171 - PubMed
  83. J Biol Chem. 1994 Oct 14;269(41):25646-54 - PubMed
  84. Cell Tissue Res. 2012 Apr;348(1):189-97 - PubMed
  85. J Thromb Haemost. 2005 Jan;3(1):35-45 - PubMed
  86. Cytotherapy. 2004;6(6):543-53 - PubMed
  87. Prenat Diagn. 2010 Jul;30(7):653-67 - PubMed
  88. Thromb Res. 2010 Apr;125 Suppl 2:S36-8 - PubMed
  89. Med Sci Monit. 2007 Jun;13(6):RA87-90 - PubMed
  90. Neuroscience. 2013 Mar 27;234:158-72 - PubMed
  91. J Leukoc Biol. 2006 May;79(5):909-12 - PubMed
  92. Tissue Eng Part A. 2011 Jun;17(11-12):1549-60 - PubMed
  93. Exp Mol Med. 2013 Nov 15;45:e54 - PubMed
  94. Differentiation. 2004 Sep;72(7):319-26 - PubMed
  95. Clin Perinatol. 2014 Mar;41(1):133-48 - PubMed
  96. J Vasc Surg. 1997 Aug;26(2):302-12 - PubMed
  97. Genes Dev. 2001 Jan 1;15(1):66-78 - PubMed
  98. Fetal Diagn Ther. 2004 Nov-Dec;19(6):513-24 - PubMed
  99. Microbiol Immunol. 1992;36(8):773-89 - PubMed
  100. Mol Cell Biol. 1990 May;10(5):1969-81 - PubMed
  101. Int J Oncol. 2003 Apr;22(4):773-8 - PubMed

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