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J Food Sci Technol. 2015 Mar;52(3):1698-704. doi: 10.1007/s13197-013-1174-9. Epub 2013 Sep 27.

Qualitative test for the detection of extraneous thiocyanate in milk.

Journal of food science and technology

Prashant Kanthale, Anil Kumar, Neelam Upadhyay, Darshan Lal, Gopal Rathod, Vivek Sharma

Affiliations

  1. At/Post: Padali, Teh-Shirur (Kasar), Distt-Beed, Maharashtra India 413249.
  2. Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana India 132001.
  3. Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana India 132001 ; 41-D, M.I.G. D.D.A. Flats, Shivam Enclave, Opp. Vishwas Nagar, Delhi, India 110032.

PMID: 25745243 PMCID: PMC4348272 DOI: 10.1007/s13197-013-1174-9

Abstract

Thiocyanate is one of the three components of lactoperoxidase system for preserving the milk under the conditions where chilling facilities do not exist due to economic and technical reasons. In 1988, International Dairy Federation (IDF) has recommended the levels and the code of practices for the application of LP-system. As the excessive use of thiocyanates beyond the recommended limits can lead to human health hazards, its rapid detection in milk at the reception dock of dairy, where milk is tested for quality before its acceptance or rejection, is necessary. For simple and rapid detection of added thiocyanates in milk, two types of qualitative tests have been developed in this study, of which one is applicable directly to milk and the other is applicable to TCA filtrate of milk. The results of the test reveal that as low as 0.6 ppm of added thiocyanate (equivalent to 1 ppm of potassium thiocyanate) can be detected. The orange to orange-red color obtained in the test solution can be considered the basis of rejecting milk assuming that it has added levels of thiocyanate.

Keywords: Lactoperoxidase system; Milk; Qualitative test; Rapid detection; Thiocyanate

References

  1. J Food Sci. 2009 Mar;74(2):C170-6 - PubMed

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