Mediterr J Hematol Infect Dis. 2015 Feb 15;7(1):e2015019. doi: 10.4084/MJHID.2015.019. eCollection 2015.
Study of serum haptoglobin level and its relation to erythropoietic activity in Beta thalassemia children.
Mediterranean journal of hematology and infectious diseases
Seham M Ragab, Manal A Safan, Eman A Badr
Affiliations
Affiliations
- Departments of Pediatrics Faculty of Medicine, Menoufia University. Egypt.
- Medical Biochemistry Faculty of Medicine, Menoufia University. Egypt.
PMID: 25745546
PMCID: PMC4344174 DOI: 10.4084/MJHID.2015.019
Abstract
BACKGROUND: Serum haptoglobin (Hp) is a reliable marker for hemolysis regardless the inflammatory state.
OBJECTIVE: We investigated the possible relation between Hp depletion and hemolysis severity, hepatitis C virus (HCV) infection and iron load in β-thalassemia children.
METHODS: Twenty two β-thalassemia major (TM),20 β-thalassemia intermedia (TI) children with 20 age and sex matched healthy controls were involved. Pre-transfusion hemoglobin level was considered. Serum ferritin, Hp and transferrin receptor levels (sTfR) (by ELISA ), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (by colorimetric method) were assayed. Markers of hepatitis C virus (HCV) were done by PCR.
RESULTS: The mean Hp levels among the studied groups were as follows; 8.02 ± 0.93 (mg/dl), 8.6 ±0.72 (mg/dl) and 122 ± 18.5(mg/dl) for TM, TI and the controls respectively. Both patient groups had significantly lower Hp level compared to the controls (P<0.0001) with significant lower level in TM compared to TI children ( P= 0.034). Significant inverse correlations were found between serum Hp and sTfR levels ( reflecting the erythropoietic activity) in thalassemia children combined and in each group (TM and TI) as well as among HCV infected children. STfR was the only significant independent predictor for serum Hp level (t= -5.585, P<0.0001). Among HCV infected patients, no significant correlation was found between serum Hp and serum transaminases.
CONCLUSION: Serum Hp depletion in thalassemia had significant relation to disease severity and correlated well with their erythropoietic activity, as assessed by the measurement of sTfR without significant relation to HCV infection. Extensive multicenter studies are recommended.
References
- Haematologica. 2007 May;92(5):583-8 - PubMed
- Transfusion. 1997 Feb;37(2):135-40 - PubMed
- J Trop Pediatr. 2004 Dec;50(6):369-71 - PubMed
- Singapore Med J. 2006 Feb;47(2):138-42 - PubMed
- Semin Hematol. 2001 Oct;38(4):360-6 - PubMed
- EMBO J. 1987 Jul;6(7):1905-12 - PubMed
- Br J Haematol. 2008 Apr;141(1):100-4 - PubMed
- Eur J Clin Invest. 2006 Mar;36(3):202-9 - PubMed
- Ann Hepatol. 2013 Jan-Feb;12 (1):54-61 - PubMed
- J Biol Chem. 1990 Nov 5;265(31):19077-81 - PubMed
- Blood. 2008 Aug 1;112(3):875-85 - PubMed
- Nature. 2001 Jan 11;409(6817):198-201 - PubMed
- Gastroenterol Clin Biol. 1993;17(5):364-9 - PubMed
- Indian J Hematol Blood Transfus. 2011 Jun;27(2):65-9 - PubMed
- J Lab Clin Med. 1992 Apr;119(4):385-90 - PubMed
- Haematologica. 2003 Jun;88(6):631-6 - PubMed
- Inflamm Res. 2012 Oct;61(10):1101-6 - PubMed
- Trans R Soc Trop Med Hyg. 2008 Aug;102(8):735-42 - PubMed
- Am J Hematol. 2014 Apr;89(4):443-7 - PubMed
- Blood. 1998 Mar 15;91(6):2139-45 - PubMed
- Curr Top Hematol. 1978;1:151-92 - PubMed
- Adv Hum Genet. 1982;12:189-261, 453-4 - PubMed
- Curr Opin Hematol. 2002 Mar;9(2):123-6 - PubMed
- Clin Chem. 1997 Oct;43(10):2012-3 - PubMed
- Pediatr Clin North Am. 2002 Dec;49(6):1165-91, v - PubMed
- Adv Exp Med Biol. 1994;356:61-8 - PubMed
- Clin Chem. 1996 Oct;42(10):1722-3 - PubMed
- Ann Hematol. 2012 Jun;91(6):905-9 - PubMed
- East Mediterr Health J. 2007 Jul-Aug;13(4):780-6 - PubMed
- Haematologica. 2008 Jan;93(1):111-5 - PubMed
- Eur J Clin Chem Clin Biochem. 1997 Sep;35(9):647-54 - PubMed
- N Engl J Med. 1999 Feb 11;340(6):448-54 - PubMed
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