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Dias O, Rocha M, Ferreira EC, et al. Reconstructing genome-scale metabolic models with merlin. Nucleic Acids Res. 2015;43(8):3899-910doi: 10.1093/nar/gkv294.
Dias, O., Rocha, M., Ferreira, E. C., & Rocha, I. (2015). Reconstructing genome-scale metabolic models with merlin. Nucleic acids research, 43(8), 3899-910. https://doi.org/10.1093/nar/gkv294
Dias, Oscar, et al. "Reconstructing genome-scale metabolic models with merlin." Nucleic acids research vol. 43,8 (2015): 3899-910. doi: https://doi.org/10.1093/nar/gkv294
Dias O, Rocha M, Ferreira EC, Rocha I. Reconstructing genome-scale metabolic models with merlin. Nucleic Acids Res. 2015 Apr 30;43(8):3899-910. doi: 10.1093/nar/gkv294. Epub 2015 Apr 06. PMID: 25845595; PMCID: PMC4417185.
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Nucleic Acids Res. 2015 Apr 30;43(8):3899-910. doi: 10.1093/nar/gkv294. Epub 2015 Apr 06.

Reconstructing genome-scale metabolic models with merlin.

Nucleic acids research

Oscar Dias, Miguel Rocha, Eugénio C Ferreira, Isabel Rocha

Affiliations

  1. Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal [email protected].
  2. Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

PMID: 25845595 PMCID: PMC4417185 DOI: 10.1093/nar/gkv294

Abstract

The Metabolic Models Reconstruction Using Genome-Scale Information (merlin) tool is a user-friendly Java application that aids the reconstruction of genome-scale metabolic models for any organism that has its genome sequenced. It performs the major steps of the reconstruction process, including the functional genomic annotation of the whole genome and subsequent construction of the portfolio of reactions. Moreover, merlin includes tools for the identification and annotation of genes encoding transport proteins, generating the transport reactions for those carriers. It also performs the compartmentalisation of the model, predicting the organelle localisation of the proteins encoded in the genome and thus the localisation of the metabolites involved in the reactions promoted by such enzymes. The gene-proteins-reactions (GPR) associations are automatically generated and included in the model. Finally, merlin expedites the transition from genomic data to draft metabolic models reconstructions exported in the SBML standard format, allowing the user to have a preliminary view of the biochemical network, which can be manually curated within the environment provided by merlin.

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

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