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Elmes M, Szyszka A, Pauliat C, et al. Maternal age effects on myometrial expression of contractile proteins, uterine gene expression, and contractile activity during labor in the rat. Physiol Rep. 2015;3(4)doi: 10.14814/phy2.12305.
Elmes, M., Szyszka, A., Pauliat, C., Clifford, B., Daniel, Z., Cheng, Z., Wathes, C., & McMullen, S. (2015). Maternal age effects on myometrial expression of contractile proteins, uterine gene expression, and contractile activity during labor in the rat. Physiological reports, 3(4), . https://doi.org/10.14814/phy2.12305
Elmes, Matthew, et al. "Maternal age effects on myometrial expression of contractile proteins, uterine gene expression, and contractile activity during labor in the rat." Physiological reports vol. 3,4 (2015). doi: https://doi.org/10.14814/phy2.12305
Elmes M, Szyszka A, Pauliat C, Clifford B, Daniel Z, Cheng Z, Wathes C, McMullen S. Maternal age effects on myometrial expression of contractile proteins, uterine gene expression, and contractile activity during labor in the rat. Physiol Rep. 2015 Apr;3(4). doi: 10.14814/phy2.12305. PMID: 25876907; PMCID: PMC4425948.
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Physiol Rep. 2015 Apr;3(4). doi: 10.14814/phy2.12305.

Maternal age effects on myometrial expression of contractile proteins, uterine gene expression, and contractile activity during labor in the rat.

Physiological reports

Matthew Elmes, Alexandra Szyszka, Caroline Pauliat, Bethan Clifford, Zoe Daniel, Zhangrui Cheng, Claire Wathes, Sarah McMullen

Affiliations

  1. Division of Nutritional Sciences, University of Nottingham, Loughborough, UK [email protected].
  2. Division of Nutritional Sciences, University of Nottingham, Loughborough, UK.
  3. Royal Veterinary College, Reproduction and Development Group, Hatfield, UK.

PMID: 25876907 PMCID: PMC4425948 DOI: 10.14814/phy2.12305

Abstract

Advanced maternal age of first time pregnant mothers is associated with prolonged and dysfunctional labor and significant risk of emergency cesarean section. We investigated the influence of maternal age on myometrial contractility, expression of contractile associated proteins (CAPs), and global gene expression in the parturient uterus. Female Wistar rats either 8 (YOUNG n = 10) or 24 (OLDER n = 10) weeks old were fed laboratory chow, mated, and killed during parturition. Myometrial strips were dissected to determine contractile activity, cholesterol (CHOL) and triglycerides (TAG) content, protein expression of connexin-43 (GJA1), prostaglandin-endoperoxide synthase 2 (PTGS2), and caveolin 1 (CAV-1). Maternal plasma concentrations of prostaglandins PGE2, PGF2α, and progesterone were determined by RIA. Global gene expression in uterine samples was compared using Affymetrix Genechip Gene 2.0 ST arrays and Ingenuity Pathway analysis (IPA). Spontaneous contractility in myometrium exhibited by YOUNG rats was threefold greater than OLDER animals (P < 0.027) but maternal age had no significant effect on myometrial CAP expression, lipid profiles, or pregnancy-related hormones. OLDER myometrium increased contractile activity in response to PGF2α, phenylephrine, and carbachol, a response absent in YOUNG rats (all P < 0.002). Microarray analysis identified that maternal age affected expression of genes related to immune and inflammatory responses, lipid transport and metabolism, steroid metabolism, tissue remodeling, and smooth muscle contraction. In conclusion YOUNG laboring rat myometrium seems primed to contract maximally, whereas activity is blunted in OLDER animals and requires stimulation to meet contractile potential. Further work investigating maternal age effects on myometrial function is required with focus on lipid metabolism and inflammatory pathways.

© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Keywords: Maternal age; myometrium; parturition

References

  1. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14729-34 - PubMed
  2. PLoS One. 2012;7(3):e31680 - PubMed
  3. Immunol Rev. 1987 Aug;98:95-114 - PubMed
  4. Biol Reprod. 2002 Feb;66(2):445-9 - PubMed
  5. Gen Comp Endocrinol. 1998 Oct;112(1):89-95 - PubMed
  6. Hum Reprod. 1999 Jan;14(1):229-36 - PubMed
  7. BJOG. 2007 Mar;114(3):343-8 - PubMed
  8. Biol Chem. 2002 May;383(5):725-37 - PubMed
  9. Reprod Biol Endocrinol. 2007 Jul 06;5:28 - PubMed
  10. J Perinat Med. 2010 Nov;38(6):617-43 - PubMed
  11. Cell Tissue Res. 2011 Jun;344(3):567-76 - PubMed
  12. Mol Hum Reprod. 2003 Jan;9(1):41-5 - PubMed
  13. Genes Dev. 1995 Mar 15;9(6):639-49 - PubMed
  14. Br J Cancer. 1981 Sep;44(3):418-24 - PubMed
  15. J Biol Chem. 1998 Mar 6;273(10):5419-22 - PubMed
  16. Prostaglandins. 1996 Jul;52(1):13-34 - PubMed
  17. Mol Cell Biol. 2010 Nov;30(22):5318-24 - PubMed
  18. Reprod Biol Endocrinol. 2005 Jul 14;3:28 - PubMed
  19. Am J Reprod Immunol. 2000 May;43(5):272-7 - PubMed
  20. J Biol Chem. 1995 Feb 3;270(5):2176-82 - PubMed
  21. Am J Obstet Gynecol. 1992 Oct;167(4 Pt 1):863-72 - PubMed
  22. J Clin Endocrinol Metab. 1994 Sep;79(3):894-9 - PubMed
  23. Mediators Inflamm. 2012;2012:967629 - PubMed
  24. Clin Sci (Lond). 2004 Jan;106(1):93-8 - PubMed
  25. Eur J Obstet Gynecol Reprod Biol. 2003 Nov 28;111 Suppl 1:S45-52 - PubMed
  26. Auton Neurosci. 2000 Nov 1;84(3):176-85 - PubMed
  27. Free Radic Biol Med. 1999 Nov;27(9-10):951-65 - PubMed
  28. Reproduction. 2007 Dec;134(6):811-22 - PubMed
  29. Acta Obstet Gynecol Scand. 2002 Apr;81(4):313-6 - PubMed
  30. J Muscle Res Cell Motil. 2012 Aug;33(3-4):209-17 - PubMed
  31. Biochem J. 1984 Jun 1;220(2):507-12 - PubMed
  32. Reprod Biol Endocrinol. 2006 May 04;4:24 - PubMed
  33. J Physiol. 2006 Jan 1;570(Pt 1):29-35 - PubMed
  34. Res Commun Chem Pathol Pharmacol. 1984 Jul;45(1):157-60 - PubMed
  35. J Neuroimmunol. 2005 May;162(1-2):190-3 - PubMed
  36. Biochim Biophys Acta. 2004 Nov 8;1686(1-2):139-47 - PubMed
  37. J Steroid Biochem Mol Biol. 2013 Sep;137:176-82 - PubMed
  38. Am J Obstet Gynecol. 2006 Aug;195(2):394.e1-24 - PubMed
  39. Genes Dev. 2001 Oct 1;15(19):2598-612 - PubMed
  40. Biol Reprod. 1992 Jan;46(1):58-64 - PubMed
  41. J Urol. 2001 Aug;166(2):734-8 - PubMed
  42. J Biol Chem. 1998 Feb 20;273(8):4574-84 - PubMed
  43. J Soc Gynecol Investig. 2003 Sep;10(6):323-38 - PubMed
  44. Steroids. 1997 Jan;62(1):143-7 - PubMed
  45. Am J Physiol Cell Physiol. 2005 Sep;289(3):C697-707 - PubMed
  46. J Cell Sci. 2006 Dec 15;119(Pt 24):5011-4 - PubMed
  47. J Exp Med. 2004 Feb 16;199(4):525-33 - PubMed
  48. Obstet Gynecol. 2001 Feb;97(2):235-42 - PubMed
  49. Am J Obstet Gynecol. 1995 Dec;173(6):1859-64 - PubMed
  50. Annu Rev Immunol. 1992;10:411-52 - PubMed
  51. Am J Physiol Heart Circ Physiol. 2005 May;288(5):H2457-64 - PubMed
  52. Gynecol Obstet Invest. 2011;72 (1):37-42 - PubMed
  53. BJOG. 2001 Sep;108(9):910-8 - PubMed
  54. Prostaglandins. 1985 May;29(5):739-45 - PubMed
  55. Cochrane Database Syst Rev. 2005 Apr 18;(2):CD001992 - PubMed
  56. Ann N Y Acad Sci. 1997 Sep 26;828:254-67 - PubMed
  57. Bull Exp Biol Med. 2001 Oct;132(4):963-5 - PubMed
  58. Endocrinology. 2005 May;146(5):2388-96 - PubMed
  59. Exp Biol Med (Maywood). 2001 Jun;226(6):498-506 - PubMed
  60. Blood. 2011 Oct 13;118(15):4231-8 - PubMed
  61. Mol Hum Reprod. 2007 May;13(5):333-41 - PubMed
  62. Obstet Gynecol. 2003 Oct;102(4):791-800 - PubMed
  63. Exp Cell Res. 1998 Jul 10;242(1):18-26 - PubMed
  64. Placenta. 2001 Sep-Oct;22(8-9):663-72 - PubMed
  65. Mol Hum Reprod. 2000 Apr;6(4):375-81 - PubMed
  66. Biol Reprod. 2003 Jan;68(1):199-206 - PubMed
  67. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13015-20 - PubMed
  68. Arch Biochem Biophys. 2001 Jun 1;390(1):19-27 - PubMed
  69. J Soc Gynecol Investig. 2001 Sep-Oct;8(5):266-76 - PubMed
  70. J Clin Endocrinol Metab. 2001 Dec;86(12):5934-43 - PubMed
  71. Eur J Obstet Gynecol Reprod Biol. 2005 Oct 1;122(2):172-6 - PubMed
  72. Am J Physiol. 1998 Nov;275(5 Pt 1):L843-51 - PubMed
  73. J Immunol. 2010 Sep 15;185(6):3750-8 - PubMed
  74. Curr Opin Obstet Gynecol. 2007 Apr;19(2):110-2 - PubMed
  75. Nat Med. 2014 Apr;20(4):377-84 - PubMed
  76. J Perinat Med. 2007;35(5):385-93 - PubMed
  77. Eur J Immunol. 2010 Mar;40(3):722-32 - PubMed
  78. PLoS Med. 2008 Jul 1;5(7):e144 - PubMed
  79. Endocrinology. 1979 Sep;105(3):745-53 - PubMed
  80. Redox Biol. 2013 May 23;1:319-31 - PubMed
  81. Baillieres Clin Obstet Gynaecol. 1993 Sep;7(3):523-52 - PubMed
  82. Annu Rev Immunol. 1998;16:27-55 - PubMed
  83. Cell Biol Int. 2011 Oct;35(10):1043-53 - PubMed
  84. J Reprod Dev. 2004 Apr;50(2):215-25 - PubMed
  85. J Clin Invest. 1995 Jan;95(1):13-9 - PubMed
  86. Nat Genet. 1996 Jun;13(2):183-8 - PubMed
  87. J Leukoc Biol. 2010 Oct;88(4):625-33 - PubMed
  88. Thromb Haemost. 1995 May;73(5):798-804 - PubMed
  89. Exp Cell Res. 2004 Feb 1;293(1):31-42 - PubMed
  90. Am J Physiol Gastrointest Liver Physiol. 2007 Jul;293(1):G240-9 - PubMed
  91. Science. 2001 Sep 28;293(5539):2449-52 - PubMed
  92. Am J Obstet Gynecol. 2007 Oct;197(4):428.e1-7 - PubMed
  93. Mol Hum Reprod. 2003 May;9(5):279-90 - PubMed
  94. Am J Physiol Renal Physiol. 2011 Dec;301(6):F1143-59 - PubMed
  95. Immunity. 2001 Aug;15(2):237-47 - PubMed
  96. J Reprod Fertil. 1986 Sep;78(1):167-83 - PubMed
  97. Am J Obstet Gynecol. 2004 Mar;190(3):693-701 - PubMed
  98. Natl Vital Stat Rep. 2009 Oct 14;58(4):1-14 - PubMed
  99. Z Geburtshilfe Neonatol. 1998 Jul-Aug;202(4):172-5 - PubMed
  100. Am J Physiol Regul Integr Comp Physiol. 2000 Jun;278(6):R1415-23 - PubMed
  101. J Clin Endocrinol Metab. 2003 Sep;88(9):4432-9 - PubMed
  102. Reprod Sci. 2014 Jan;21(1):122-30 - PubMed
  103. J Exp Med. 2003 Nov 3;198(9):1289-300 - PubMed
  104. BMC Pregnancy Childbirth. 2007 Jun 01;7 Suppl 1:S10 - PubMed
  105. PLoS One. 2012;7(7):e41708 - PubMed
  106. Reprod Fertil Dev. 1993;5(1):39-48 - PubMed
  107. Hum Reprod. 2007 May;22(5):1264-72 - PubMed
  108. Curr Opin Immunol. 2003 Oct;15(5):544-52 - PubMed
  109. J Biol Chem. 1999 Oct 8;274(41):29251-9 - PubMed
  110. J Endocrinol. 2001 Mar;168(3):497-508 - PubMed
  111. Biol Reprod. 2002 Sep;67(3):1013-7 - PubMed
  112. Nat Med. 2003 Feb;9(2):213-9 - PubMed
  113. J Cell Sci. 2006 May 1;119(Pt 9):1715-22 - PubMed
  114. Thromb Haemost. 1996 Aug;76(2):220-5 - PubMed
  115. Mol Cell. 2004 Nov 5;16(3):331-41 - PubMed
  116. J Biol Chem. 2003 Mar 21;278(12 ):10443-9 - PubMed
  117. Int J Prev Med. 2013 Jun;4(6):624-30 - PubMed
  118. Cell Signal. 1998 Jul;10(7):457-63 - PubMed
  119. Biol Reprod. 2007 Aug;77(2):190-201 - PubMed
  120. Biochem Biophys Res Commun. 2007 Jun 22;358(1):66-72 - PubMed
  121. Am J Obstet Gynecol. 2000 Jun;182(6):1312-20 - PubMed
  122. J Anat. 2005 Aug;207(2):125-34 - PubMed
  123. BMC Pregnancy Childbirth. 2007 Jun 01;7 Suppl 1:S7 - PubMed
  124. Mol Cell Endocrinol. 2011 Mar 15;335(1):2-13 - PubMed
  125. Free Radic Res. 2006 May;40(5):495-505 - PubMed
  126. Biochimie. 2006 Mar-Apr;88(3-4):285-95 - PubMed
  127. Fertil Steril. 2008 Dec;90(6):2340-50 - PubMed
  128. Natl Vital Stat Rep. 2006 Sep 29;55(1):1-101 - PubMed
  129. Biochim Biophys Acta. 1991 Sep 24;1094(3):339-45 - PubMed
  130. Hum Reprod. 2011 Apr;26(4):748-57 - PubMed
  131. Nutr Metab Cardiovasc Dis. 2013 Oct;23(10):967-72 - PubMed
  132. J Membr Biol. 2004 Mar 15;198(2):95-101 - PubMed
  133. J Endocrinol. 1998 Dec;159(3):519-26 - PubMed
  134. BJOG. 2001 Jan;108(1):48-55 - PubMed
  135. Am J Physiol Cell Physiol. 2005 May;288(5):C982-8 - PubMed

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