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Luo TL, Rickard AH, Srinivasan U, et al. Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system. Front Microbiol. 2015;6:182doi: 10.3389/fmicb.2015.00182.
Luo, T. L., Rickard, A. H., Srinivasan, U., Kaye, K. S., & Foxman, B. (2015). Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system. Frontiers in microbiology, 6182. https://doi.org/10.3389/fmicb.2015.00182
Luo, Ting L, et al. "Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system." Frontiers in microbiology vol. 6 (2015): 182. doi: https://doi.org/10.3389/fmicb.2015.00182
Luo TL, Rickard AH, Srinivasan U, Kaye KS, Foxman B. Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system. Front Microbiol. 2015 Mar 12;6:182. doi: 10.3389/fmicb.2015.00182. eCollection 2015. PMID: 25814985; PMCID: PMC4357298.
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Front Microbiol. 2015 Mar 12;6:182. doi: 10.3389/fmicb.2015.00182. eCollection 2015.

Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system.

Frontiers in microbiology

Ting L Luo, Alexander H Rickard, Usha Srinivasan, Keith S Kaye, Betsy Foxman

Affiliations

  1. Department of Epidemiology, School of Public Health, University of Michigan Ann Arbor, MI, USA.
  2. Division of Infectious Diseases, Wayne State University Detroit, MI, USA.

PMID: 25814985 PMCID: PMC4357298 DOI: 10.3389/fmicb.2015.00182

Abstract

OBJECTIVES: Acinetobacter baumannii is an emerging opportunistic nosocomial pathogen. Two factors that may enhance persistence in healthcare settings are antimicrobial resistance and biofilm-forming ability. The aim of this work was to determine whether A. baumannii isolates that persist in healthcare settings (endemic), can be differentiated from sporadic isolates based upon their ability to resist antibiotics and their biofilm-forming capability.

METHODS: Two hundred and ninety A. baumannii isolates were isolated over 17 months in the Detroit Medical Center (DMC). The isolates were genotyped using repetitive extragenic palindromic-PCR (REP-PCR). REP-types appearing greater than 10 times during active surveillance were considered endemic. The in vitro biofilm-forming ability and antibiotic resistance profile of each isolate were evaluated. Isolates were tested for the presence of two genetic markers-one implicated in biofilm formation (bap) and the other in antibiotic resistance (blaOXA-23).

RESULTS: Of the 290 isolates evaluated, 84% carried bap and 36% carried blaOXA-23 . Five unique REP-PCR banding-types were detected >10 times (endemic) and constituted 58% of the 290 isolates. These five endemic REP-PCR types were 5.1 times more likely than sporadic isolates to carry both bap and blaOXA-23 . Furthermore, endemic isolates were resistant to 3 more antibiotic classes, on average, than sporadic isolates and four of the five endemic REP-PCR types formed denser biofilms in vitro than sporadic isolates.

CONCLUSIONS: Endemic A. baumannii isolates are more likely than sporadic isolates to possess factors that increase virulence and enhance survival within a large healthcare system.

Keywords: OXA-23; REP-PCR; antibiotic resistance; bap; biofilms; molecular epidemiology

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