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Wang Z, Tonderys D, Leggett SE, et al. Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology. Carbon N Y. 2016;97:14-24doi: 10.1016/j.carbon.2015.03.040.
Wang, Z., Tonderys, D., Leggett, S. E., Williams, E. K., Kiani, M. T., Steinberg, R. S., Qiu, Y., Wong, I. Y., & Hurt, R. H. (2016). Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology. Carbon, 9714-24. https://doi.org/10.1016/j.carbon.2015.03.040
Wang, Zhongying, et al. "Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology." Carbon vol. 97 (2016): 14-24. doi: https://doi.org/10.1016/j.carbon.2015.03.040
Wang Z, Tonderys D, Leggett SE, Williams EK, Kiani MT, Steinberg RS, Qiu Y, Wong IY, Hurt RH. Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology. Carbon N Y. 2016 Feb 01;97:14-24. doi: 10.1016/j.carbon.2015.03.040. PMID: 25848137; PMCID: PMC4384125.
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Carbon N Y. 2016 Feb 01;97:14-24. doi: 10.1016/j.carbon.2015.03.040.

Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology.

Carbon

Zhongying Wang, Daniel Tonderys, Susan E Leggett, Evelyn Kendall Williams, Mehrdad T Kiani, Ruben Spitz Steinberg, Yang Qiu, Ian Y Wong, Robert H Hurt

Affiliations

  1. School of Engineering, Brown University, Providence, RI 02912 ; Department of Chemistry, Brown University, Providence, RI 02912.
  2. School of Engineering, Brown University, Providence, RI 02912 ; Center for Biomedical Engineering, Brown University, Providence, RI 02912.
  3. School of Engineering, Brown University, Providence, RI 02912 ; Center for Biomedical Engineering, Brown University, Providence, RI 02912 ; Pathobiology Graduate Program, Brown University, Providence, RI 02912.
  4. School of Engineering, Brown University, Providence, RI 02912.
  5. School of Engineering, Brown University, Providence, RI 02912 ; Center for Biomedical Engineering, Brown University, Providence, RI 02912 ; Pathobiology Graduate Program, Brown University, Providence, RI 02912 ; Institute for Molecular and Nanoscale Innovation, Brown University, Providence, RI 02912.
  6. School of Engineering, Brown University, Providence, RI 02912 ; Center for Biomedical Engineering, Brown University, Providence, RI 02912 ; Institute for Molecular and Nanoscale Innovation, Brown University, Providence, RI 02912.

PMID: 25848137 PMCID: PMC4384125 DOI: 10.1016/j.carbon.2015.03.040

Abstract

Textured surfaces with periodic topographical features and long-range order are highly attractive for directing cell-material interactions. They mimic physiological environments more accurately than planar surfaces and can fundamentally alter cell alignment, shape, gene expression, and cellular assembly into superstructures or microtissues. Here we demonstrate for the first time that wrinkled graphene-based surfaces are suitable as textured cell attachment substrates, and that engineered wrinkling can dramatically alter cell alignment and morphology. The wrinkled surfaces are fabricated by graphene oxide wet deposition onto pre-stretched elastomers followed by relaxation and mild thermal treatment to stabilize the films in cell culture medium. Multilayer graphene oxide films form periodic, delaminated buckle textures whose wavelengths and amplitudes can be systematically tuned by variation in the wet deposition process. Human and murine fibroblasts attach to these textured films and remain viable, while developing pronounced alignment and elongation relative to those on planar graphene controls. Compared to lithographic patterning of nanogratings, this method has advantages in the simplicity and scalability of fabrication, as well as the opportunity to couple the use of topographic cues with the unique conductive, adsorptive, or barrier properties of graphene materials for functional biomedical devices.

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