Iran J Microbiol. 2013 Dec;5(4):315-22.
Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran.
Iranian journal of microbiology
Saeed Shoja, Mojtaba Moosavian, Amir Peymani, Mohammad Amin Tabatabaiefar, Soodabeh Rostami, Nasim Ebrahimi
Affiliations
Affiliations
- Department of Microbiology, School of Medicine, Infectious & Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
- Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran.
- Genetic Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
PMID: 25848498
PMCID: PMC4385154
Abstract
BACKGROUND AND OBJECTIVES: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units.
MATERIALS AND METHODS: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method.
RESULTS: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%).
CONCLUSION: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.
Keywords: Acinetobacter baumannii; Carbapenem resistant; Intensive Care Units
References
- Transplant Proc. 2011 Oct;43(8):3116-20 - PubMed
- Int J Antimicrob Agents. 2011 Nov;38(5):403-8 - PubMed
- Int J Antimicrob Agents. 2012 Jul;40(1):89-91 - PubMed
- Lung India. 2010 Oct;27(4):217-20 - PubMed
- J Antimicrob Chemother. 2011 Jan;66(1):54-61 - PubMed
- J Clin Microbiol. 1996 May;34(5):1193-202 - PubMed
- Int J Antimicrob Agents. 2008 Jan;31(1):50-4 - PubMed
- Int J Antimicrob Agents. 2010 Aug;36(2):114-8 - PubMed
- Int J Antimicrob Agents. 2010 Mar;35(3):269-73 - PubMed
- Int J Antimicrob Agents. 2009 Jul;34(1):25-8 - PubMed
- J Hosp Infect. 2011 Jun;78(2):143-8 - PubMed
- Diagn Microbiol Infect Dis. 2008 Nov;62(3):326-32 - PubMed
- J Infect Dev Ctries. 2009 Jun 01;3(5):335-41 - PubMed
- Int J Antimicrob Agents. 2012 Feb;39(2):105-14 - PubMed
- Scand J Infect Dis. 2013 Jan;45(1):26-31 - PubMed
- Diagn Microbiol Infect Dis. 2011 May;70(1):119-23 - PubMed
- Int J Antimicrob Agents. 2006 Apr;27(4):351-3 - PubMed
- Int J Antimicrob Agents. 2011 Apr;37(4):302-8 - PubMed
- Int J Antimicrob Agents. 2012 Jun;39(6):526-8 - PubMed
- J Clin Microbiol. 2002 Dec;40(12):4571-5 - PubMed
- Int J Antimicrob Agents. 2008 Jul;32(1):29-32 - PubMed
- Int J Infect Dis. 2010 Aug;14(8):e723-9 - PubMed
- Int J Antimicrob Agents. 2012 Nov;40(5):398-403 - PubMed
- Clin Microbiol Infect. 2000 Dec;6(12):635-43 - PubMed
- Euro Surveill. 2012 Feb 16;17(7):null - PubMed
- Iran J Microbiol. 2011 Jun;3(2):68-74 - PubMed
- Iran J Microbiol. 2011 Dec;3(4):162-9 - PubMed
- Jpn J Infect Dis. 2011;64(1):69-71 - PubMed
- Intensive Care Med. 2005 May;31(5):649-55 - PubMed
- J Antimicrob Chemother. 2010 Oct;65(10):2253-4 - PubMed
- Jpn J Infect Dis. 2008 Jul;61(4):274-8 - PubMed
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