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Strong AL, Bowles AC, MacCrimmon CP, et al. Characterization of a Murine Pressure Ulcer Model to Assess Efficacy of Adipose-derived Stromal Cells. Plast Reconstr Surg Glob Open. 2015;3(3):e334doi: 10.1097/GOX.0000000000000260.
Strong, A. L., Bowles, A. C., MacCrimmon, C. P., Lee, S. J., Frazier, T. P., Katz, A. J., Gawronska-Kozak, B., Bunnell, B. A., & Gimble, J. M. (2015). Characterization of a Murine Pressure Ulcer Model to Assess Efficacy of Adipose-derived Stromal Cells. Plastic and reconstructive surgery. Global open, 3(3), e334. https://doi.org/10.1097/GOX.0000000000000260
Strong, Amy L, et al. "Characterization of a Murine Pressure Ulcer Model to Assess Efficacy of Adipose-derived Stromal Cells." Plastic and reconstructive surgery. Global open vol. 3,3 (2015): e334. doi: https://doi.org/10.1097/GOX.0000000000000260
Strong AL, Bowles AC, MacCrimmon CP, Lee SJ, Frazier TP, Katz AJ, Gawronska-Kozak B, Bunnell BA, Gimble JM. Characterization of a Murine Pressure Ulcer Model to Assess Efficacy of Adipose-derived Stromal Cells. Plast Reconstr Surg Glob Open. 2015 Apr 07;3(3):e334. doi: 10.1097/GOX.0000000000000260. eCollection 2015 Mar. PMID: 25878945; PMCID: PMC4387156.
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Plast Reconstr Surg Glob Open. 2015 Apr 07;3(3):e334. doi: 10.1097/GOX.0000000000000260. eCollection 2015 Mar.

Characterization of a Murine Pressure Ulcer Model to Assess Efficacy of Adipose-derived Stromal Cells.

Plastic and reconstructive surgery. Global open

Amy L Strong, Annie C Bowles, Connor P MacCrimmon, Stephen J Lee, Trivia P Frazier, Adam J Katz, Barbara Gawronska-Kozak, Bruce A Bunnell, Jeffrey M Gimble

Affiliations

  1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, La.; Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Florida, Gainesville, Fla.; Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland; Department of Pharmacology, Tulane University School of Medicine, New Orleans, La.; LaCell LLC, New Orleans, La.; Department of Medicine, Tulane Health Sciences Center, New Orleans, La.; and Department of Surgery, Tulane Health Sciences Center, New Orleans, La.

PMID: 25878945 PMCID: PMC4387156 DOI: 10.1097/GOX.0000000000000260

Abstract

BACKGROUND: As the world's population lives longer, the number of individuals at risk for pressure ulcers will increase considerably in the coming decades. In developed countries, up to 18% of nursing home residents suffer from pressure ulcers and the resulting hospital costs can account for up to 4% of a nation's health care budget. Although full-thickness surgical skin wounds have been used as a model, preclinical rodent studies have demonstrated that repeated cycles of ischemia and reperfusion created by exposure to magnets most closely mimic the human pressure ulcer condition.

METHODS: This study uses in vivo and in vitro quantitative parameters to characterize the temporal kinetics and histology of pressure ulcers in young, female C57BL/6 mice exposed to 2 or 3 ischemia-reperfusion cycles. This pressure ulcer model was validated further in studies examining the efficacy of adipose-derived stromal/stem cell administration.

RESULTS: Optimal results were obtained with the 2-cycle model based on the wound size, histology, and gene expression profile of representative angiogenic and reparative messenger RNAs. When treated with adipose-derived stromal/stem cells, pressure ulcer wounds displayed a dose-dependent and significant acceleration in wound closure rates and improved tissue histology.

CONCLUSION: These findings document the utility of this simplified preclinical model for the evaluation of novel tissue engineering and medical approaches to treat pressure ulcers in humans.

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