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Martucci NM, Rea I, Ruggiero I, et al. A new strategy for label-free detection of lymphoma cancer cells. Biomed Opt Express. 2015;6(4):1353-62doi: 10.1364/BOE.6.001353.
Martucci, N. M., Rea, I., Ruggiero, I., Terracciano, M., De Stefano, L., Migliaccio, N., Palmieri, C., Scala, G., Arcari, P., Rendina, I., & Lamberti, A. (2015). A new strategy for label-free detection of lymphoma cancer cells. Biomedical optics express, 6(4), 1353-62. https://doi.org/10.1364/BOE.6.001353
Martucci, Nicola M, et al. "A new strategy for label-free detection of lymphoma cancer cells." Biomedical optics express vol. 6,4 (2015): 1353-62. doi: https://doi.org/10.1364/BOE.6.001353
Martucci NM, Rea I, Ruggiero I, Terracciano M, De Stefano L, Migliaccio N, Palmieri C, Scala G, Arcari P, Rendina I, Lamberti A. A new strategy for label-free detection of lymphoma cancer cells. Biomed Opt Express. 2015 Mar 19;6(4):1353-62. doi: 10.1364/BOE.6.001353. eCollection 2015 Apr 01. PMID: 25909019; PMCID: PMC4399674.
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Biomed Opt Express. 2015 Mar 19;6(4):1353-62. doi: 10.1364/BOE.6.001353. eCollection 2015 Apr 01.

A new strategy for label-free detection of lymphoma cancer cells.

Biomedical optics express

Nicola M Martucci, Ilaria Rea, Immacolata Ruggiero, Monica Terracciano, Luca De Stefano, Nunzia Migliaccio, Camillo Palmieri, Giuseppe Scala, Paolo Arcari, Ivo Rendina, Annalisa Lamberti

Affiliations

  1. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
  2. Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy.
  3. Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy ; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy.
  4. Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Viale Europa, 88100 Germaneto, Catanzaro, Italy.

PMID: 25909019 PMCID: PMC4399674 DOI: 10.1364/BOE.6.001353

Abstract

In this paper, a new strategy for highly selective and sensitive direct detection of lymphoma cells by exploiting the interaction between a peptide and its B-cell receptor, has been evaluated. In particular, an idiotype peptide, able to specifically bind the B-cell receptor of A20 cells in mice engrafted with A20 lymphoma, has been used as molecular probe. The new detection technique has been demonstrated on a planar crystalline silicon chip. Coverage of 85% of silicon surface and detection efficiency of 8.5 × 10(-3) cells/μm(2) were obtained. The recognition strategy promises to extend its application in studying the interaction between ligands and their cell-surface receptors.

Keywords: (000.1430) Biology and medicine; (280.1415) Biological sensing and sensors

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