J Diabetes Metab Disord. 2015 Mar 19;14:14. doi: 10.1186/s40200-015-0142-5. eCollection 2015.
Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats.
Journal of diabetes and metabolic disorders
Seyed Jafar Hashemian, Mojtaba Rismanchi, Ensiyeh Nasli Esfahani, Amir Khoshvaghti, Farideh Razi
Affiliations
Affiliations
- Diabetes Research Center, Endocrinology and Metabolism Clinical Science Institute, Tehran University of Medical Sciences, Tehran, Iran.
- Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran.
- Faculty of Aerospace and Sub-Aquatic Medicine, AJA University of Medical Sciences, Tehran, Iran.
PMID: 25806360
PMCID: PMC4371718 DOI: 10.1186/s40200-015-0142-5
Abstract
BACKGROUND: Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not examined yet in space flight models of osteoporosis in rats.
METHODS: This was an interventional animal study being performed in a 1-month period. We included 21 Sprague Dawley strain rats (>200 gr, >6 week) who were randomly assigned to receive daily supplementation of oral 0.03μgr calcitriol and to be submitted to tail suspension model. Rats were followed for 1 month and were tested for serum osteocalcin (OC), alkaline phosphatase (ALP) and serum calcium at the beginning and the end of the study period. The results were analyzed and compared between groups.
RESULTS: Although serum levels of osteocalcin and alkaline phosphatase biomarkers and total serum calcium were not significantly different within and between study groups, their levels were increased in tail suspension model compared to control group. The levels of these biomarkers were lower in those who were submitted to tail suspension model and received calcitriol supplementation compared to those who were only submitted to tail suspension (60.14 ± 11.73 ng/mL vs. 58.29 ± 2.69 ng/mL; p = 0.696 for osteocalcin and 381.86 ± 99.16 mU/mL vs. 362.57 ± 27.41 ng/mL; p = 0.635 for alkaline phosphatase).
CONCLUSION: Supplementation of daily diet with calcitriol in rats under weightlessness conditions may results in lower values for bone metabolic biomarkers of alkaline phosphatase and osteocalcin and serum calcium. This pattern of change in biomarkers of bone formation, may point to the capacity of calcitriol supplementation in preventing cellular process of osteoporosis. Thus calcitriol supplementation could be an available, economic and effective strategy for preventing bone metabolic changes related to weightlessness commonly encountered in space flight. The outcome of this study needs to be further studied in future trying to find more definite results.
Keywords: Alkaline phosphatase (ALP); Calcitriol; Osteocalcin (OC); Tail suspension model; Vitamin D
References
- Bone. 1998 May;22(5 Suppl):83S-88S - PubMed
- Ann Intern Med. 1984 Jan;100(1):1-6 - PubMed
- Acta Astronaut. 1995 Oct-Dec;36(8-12):463-6 - PubMed
- Lancet. 2007 Aug 25;370(9588):657-66 - PubMed
- Gravit Space Biol Bull. 2005 Jun;18(2):39-58 - PubMed
- J Bone Miner Res. 2003 Mar;18(3):561-9 - PubMed
- J Bone Miner Metab. 2002;20(4):216-22 - PubMed
- J Bone Miner Res. 2005 Jun;20(6):962-70 - PubMed
- Adv Space Biol Med. 2005;10:7-40 - PubMed
- J Clin Endocrinol Metab. 2001 Mar;86(3):1212-21 - PubMed
- Keio J Med. 2005 Jun;54(2):55-9 - PubMed
- J Clin Endocrinol Metab. 2013 Oct;98(10):4023-9 - PubMed
- Curr Osteoporos Rep. 2013 Jun;11(2):92-8 - PubMed
- Hepatology. 1998 Sep;28(3):695-9 - PubMed
- N Engl J Med. 2006 Feb 16;354(7):669-83 - PubMed
- PLoS One. 2013 Jun 20;8(6):e66991 - PubMed
- J Natl Cancer Inst. 2005 Jan 5;97(1):59-69 - PubMed
- Chin Med J (Engl). 2012 Nov;125(22):4093-7 - PubMed
- BMJ. 2010 Jan 11;340:b5664 - PubMed
- Endocrinol Metab Clin North Am. 1990 Mar;19(1):1-18 - PubMed
- Hemodial Int. 2010 Apr;14 (2):182-92 - PubMed
- J Appl Physiol (1985). 1999 Jun;86(6):1841-6 - PubMed
- J Bone Miner Res. 2013 Jun;28(6):1243-55 - PubMed
- J Bone Miner Res. 2005 Feb;20(2):208-18 - PubMed
- Br J Nutr. 2010 Oct;104(8):1091-5 - PubMed
- J Appl Physiol (1985). 2002 Apr;92(4):1367-77 - PubMed
- Aviat Space Environ Med. 1987 Jan;58(1):63-8 - PubMed
- Bone. 1998 May;22(5 Suppl):105S-111S - PubMed
- J Pharmacol Exp Ther. 1999 Oct;291(1):321-8 - PubMed
- Clin Endocrinol (Oxf). 2001 Feb;54(2):219-24 - PubMed
- Clin Rheumatol. 1995 Jul;14(4):420-4 - PubMed
- J Musculoskelet Neuronal Interact. 2001 Mar;1(3):193-207 - PubMed
- Lancet. 2000 May 6;355(9215):1607-11 - PubMed
- Lancet. 2000 May 6;355(9215):1569-70 - PubMed
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