Display options
Cite
Ojeda JE, Cardenas G, Klassen R, et al. Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films . Wounds. 2011;23(5):135-43
Ojeda, J. E., Cardenas, G., Klassen, R., von Plessing, C., Grez, P., & Sepulveda, J. (2011). Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films . Wounds : a compendium of clinical research and practice, 23(5), 135-43.
Ojeda, Javier E, et al. "Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films ." Wounds : a compendium of clinical research and practice vol. 23,5 (2011): 135-43.
Ojeda JE, Cardenas G, Klassen R, von Plessing C, Grez P, Sepulveda J. Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films . Wounds. 2011 May;23(5):135-43. PMID: 25881360.
Copy Download .nbib Format: NLM
Share it on

Wounds. 2011 May;23(5):135-43.

Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films .

Wounds : a compendium of clinical research and practice

Javier E Ojeda, Galo Cardenas, Rodrigo Klassen, Carlos von Plessing, Patricia Grez, Jacqueline Sepulveda

Affiliations

  1. ?Institute of Clinical Veterinary Sciences, University of Southern Chile; Email; [email protected].
  2. ?

PMID: 25881360

Abstract

UNLABELLED:  Nitric oxide (NO) signaling appears to play a vital role in wound healing associated to improve collagen and angiogenesis. A burn wound model was used to evaluate the effects of a chitosan films on histopathological features, nitric oxide synthase (NOS) activity, and quantification of neoformed capillaries assessed with CD34.

METHODS: Bilateral burns (n = 16) were made on adult Sprague-Dawley rats. The lesions on the right side of the rats were treated with chitosan films, and the lesions on the left side of the same rats were treated with gauze with NaCl 0.9% as a control.

RESULTS: Histological analysis revealed accelerated burn wound healing supported by significant differences in acute inflammation, collagen, and granulation tissue formation in chitosan-treated burns. Additionally, chitosan-treated burns were associated with higher CD34 immunoreactivity antibody supported by significant differences. This analysis of NOS activity was statistically significant on treated burns in the second treatment week. NOS results are associated with the highest collagen deposition, granulation tissue formation, and new capillary formation.

CONCLUSION: The use of chitosan on burns promoted re-epithelialization by means of angiogenic and NO release associated with higher cell infiltration into the wound bed during the proliferative phase.

Publication Types